원형질체 재생을 통한 느티만가닥버섯 단핵균주 선발Selection of parental monokaryons from Korean Hypsizigus marmoreus by protoplast regeneration
- Other Titles
- Selection of parental monokaryons from Korean Hypsizigus marmoreus by protoplast regeneration
- Authors
- 오연이; 공원식; 장갑열; 신평균; 김은선; 오민지; 최인걸
- Issue Date
- 2015
- Publisher
- 한국버섯학회
- Keywords
- Hypsigus marmoreus; Parental monokaryons; Protoplast isolation and regeneration
- Citation
- 한국버섯학회지, v.13, no.3, pp.270 - 273
- Indexed
- KCI
OTHER
- Journal Title
- 한국버섯학회지
- Volume
- 13
- Number
- 3
- Start Page
- 270
- End Page
- 273
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/95526
- ISSN
- 1738-0294
- Abstract
- Hypsizigus marmoreus is commercially the most important edible mushroom in Japan. This mushroom is usually cultivated for a longer period (about 85~120 days) than other mushroom. In order to develop a new cultivar that has a shortened cultivation period, the genome analysis of this strain has been considered. This study aims to obtain parental monokaryotic strains reproducing ‘Haemi’ cultivar in Hypsizigus marmoreus for reference genome sequencing. The mycelia were cultured in MCM and MYG media for various incubation periods. Homogenized mycelia were treated with commercial cell wall degrading enzymes to maximize protoplasts production yield from Hypsizigus marmoreus. The greatest number of protoplasts was obtained from mycelia cultured in MCM media for 3 days using Novozyme enzyme. The isolated protoplasts were grown in regeneration agar media after two weeks. Regenerated colonies were picked and moved on separated dishes for microscopic observation. Neohaplonts regenerated from dikayotic strains were identified by the absence of clamp connections. We confirmed that one of monokaryotic strains is a parental strain by crossing with an original compatible strain of ‘Haemi’ cultivar. This parental strain will be used for reference genome sequence analysis.
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