Chemical properties and immuno-stimulating activities of crude polysaccharides from enzyme digests of tea leaves
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, H.-R. | - |
dc.contributor.author | Suh, H.J. | - |
dc.contributor.author | Yu, K.-W. | - |
dc.contributor.author | Kim, T.Y. | - |
dc.contributor.author | Shin, K.-S. | - |
dc.date.accessioned | 2021-09-05T00:00:25Z | - |
dc.date.available | 2021-09-05T00:00:25Z | - |
dc.date.created | 2021-06-17 | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1226-3311 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/95948 | - |
dc.description.abstract | In order to develop new immuno-stimulating ingredients from mature leaves of green tea, crude polysaccharides were isolated from pectinase digests of tea leaves (green tea enzyme digestion, GTE-0), after which their immuno-stimulating activities and chemical properties were examined. GTE-0 mainly contained neutral sugars (54.9%) such as glucose (14.2%), arabinose (12.2%), rhamnose (11.1%), and galacturonic acid (45.1%), which are characteristic of pectic polysaccharides. The anti-complementary activity of GTE-0 was similar to that of polysaccharide K (used as positive control). Number of morphologically activated macrophages was significantly increased in the GTE-0-treated group. GTE-0 significantly augmented H2O2 and reactive oxygen species production by murine peritoneal macrophage cells in a dose-dependent manner, whereas production of nitric oxide showed the highest activity at a dose of 100 μg/mL among all tested concentrations. Murine peritoneal macrophages stimulated with GTE-0 showed enhanced production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factors-α in a dose-dependent manner. Further, GTE-0 induced higher phagocytic activity in a dose-dependent manner. In ex vivo assay for cytolytic activity of murine peritoneal macrophages, GTE-0-treated group showed significantly higher activity compared to the untreated group at an effector-to-target cell ratio of 20. The above results lead us to conclude that polysaccharides from leaves of green tea have a potent immuno-stimulating effect on murine peritoneal macrophage cells. © 2015, J Korean Soc Food Sci Nutr. All right reserved. | - |
dc.language | Korean | - |
dc.language.iso | ko | - |
dc.publisher | Korean Society of Food Science and Nutrition | - |
dc.title | Chemical properties and immuno-stimulating activities of crude polysaccharides from enzyme digests of tea leaves | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Suh, H.J. | - |
dc.identifier.doi | 10.3746/jkfn.2015.44.5.664 | - |
dc.identifier.scopusid | 2-s2.0-84930037283 | - |
dc.identifier.bibliographicCitation | Journal of the Korean Society of Food Science and Nutrition, v.44, no.5, pp.664 - 672 | - |
dc.relation.isPartOf | Journal of the Korean Society of Food Science and Nutrition | - |
dc.citation.title | Journal of the Korean Society of Food Science and Nutrition | - |
dc.citation.volume | 44 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 664 | - |
dc.citation.endPage | 672 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001992340 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | Complement | - |
dc.subject.keywordAuthor | Cytokine | - |
dc.subject.keywordAuthor | Green tea | - |
dc.subject.keywordAuthor | Macrophage | - |
dc.subject.keywordAuthor | Polysaccharide | - |
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