A “turn-on” fluorescent microbead sensor for detecting nitric oxide
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, L.-H. | - |
dc.contributor.author | Ahn, D.J. | - |
dc.contributor.author | Koo, E. | - |
dc.date.accessioned | 2021-09-05T00:03:42Z | - |
dc.date.available | 2021-09-05T00:03:42Z | - |
dc.date.created | 2021-06-17 | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1176-9114 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/95975 | - |
dc.description.abstract | Nitric oxide (NO) is a messenger molecule involved in numerous physical and pathological processes in biological systems. Therefore, the development of a highly sensitive material able to detect NO in vivo is a key step in treating cardiovascular and a number of types of cancer-related diseases, as well as neurological dysfunction. Here we describe the development of a fluorescent probe using microbeads to enhance the fluorescence signal. Microbeads are infused with the fluorophore, dansyl-piperazine (Ds-pip), and quenched when the fluorophore is coordinated with a rhodium (Rh)-complex, ie, Rh2(AcO-)4(Ds-pip). In contrast, they are able to fluoresce when the transition-metal complex is replaced by NO. To confirm the “on/off” mechanism for detecting NO, we investigated the structural molecular properties using the Fritz Haber Institute ab initio molecular simulations (FHI-AIMS) package. According to the binding energy calculation, NO molecules bind more strongly and rapidly with the Rh-core of the Rh-complex than with Ds-pip. This suggests that NO can bond strongly with the Rh-core and replace Ds-pip, even though Ds-pip is already near the Rh-core. However, the recovery process takes longer than the quenching process because the recovery process needs to overcome the energy barrier for formation of the transition state complex, ie, NO-(AcO-)4-(Ds-pip). Further, we confirm that the Rh-complex with the Ds-pip structure has too small an energy gap to give off visible light from the highest unoccupied molecular orbital/lowest unoccupied molecular orbital energy level. © 2015 Yang et al. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | Dove Medical Press Ltd | - |
dc.subject | dansyl chloride | - |
dc.subject | fluorescent dye | - |
dc.subject | nitric oxide | - |
dc.subject | piperazine | - |
dc.subject | rhodium | - |
dc.subject | microsphere | - |
dc.subject | nitric oxide | - |
dc.subject | ab initio calculation | - |
dc.subject | Article | - |
dc.subject | binding affinity | - |
dc.subject | controlled study | - |
dc.subject | energy yield | - |
dc.subject | fluorescence | - |
dc.subject | molecular sensor | - |
dc.subject | process development | - |
dc.subject | simulation | - |
dc.subject | structure analysis | - |
dc.subject | chemical structure | - |
dc.subject | chemistry | - |
dc.subject | Fluorescence | - |
dc.subject | Microspheres | - |
dc.subject | Molecular Structure | - |
dc.subject | Nitric Oxide | - |
dc.subject | Rhodium | - |
dc.title | A “turn-on” fluorescent microbead sensor for detecting nitric oxide | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ahn, D.J. | - |
dc.identifier.doi | 10.2147/IJN.S74924 | - |
dc.identifier.scopusid | 2-s2.0-84919707353 | - |
dc.identifier.wosid | 000346596700001 | - |
dc.identifier.bibliographicCitation | International Journal of Nanomedicine, v.10, pp.115 - 123 | - |
dc.relation.isPartOf | International Journal of Nanomedicine | - |
dc.citation.title | International Journal of Nanomedicine | - |
dc.citation.volume | 10 | - |
dc.citation.startPage | 115 | - |
dc.citation.endPage | 123 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | dansyl chloride | - |
dc.subject.keywordPlus | fluorescent dye | - |
dc.subject.keywordPlus | nitric oxide | - |
dc.subject.keywordPlus | piperazine | - |
dc.subject.keywordPlus | rhodium | - |
dc.subject.keywordPlus | microsphere | - |
dc.subject.keywordPlus | nitric oxide | - |
dc.subject.keywordPlus | ab initio calculation | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | binding affinity | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | energy yield | - |
dc.subject.keywordPlus | fluorescence | - |
dc.subject.keywordPlus | molecular sensor | - |
dc.subject.keywordPlus | process development | - |
dc.subject.keywordPlus | simulation | - |
dc.subject.keywordPlus | structure analysis | - |
dc.subject.keywordPlus | chemical structure | - |
dc.subject.keywordPlus | chemistry | - |
dc.subject.keywordPlus | Fluorescence | - |
dc.subject.keywordPlus | Microspheres | - |
dc.subject.keywordPlus | Molecular Structure | - |
dc.subject.keywordPlus | Nitric Oxide | - |
dc.subject.keywordPlus | Rhodium | - |
dc.subject.keywordAuthor | Ab initio molecular simulation | - |
dc.subject.keywordAuthor | Fluorescence | - |
dc.subject.keywordAuthor | Microbead | - |
dc.subject.keywordAuthor | Nitric oxide | - |
dc.subject.keywordAuthor | Rhodium complex | - |
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