A “turn-on” fluorescent microbead sensor for detecting nitric oxide
- Authors
- Yang, L.-H.; Ahn, D.J.; Koo, E.
- Issue Date
- 2015
- Publisher
- Dove Medical Press Ltd
- Keywords
- Ab initio molecular simulation; Fluorescence; Microbead; Nitric oxide; Rhodium complex
- Citation
- International Journal of Nanomedicine, v.10, pp.115 - 123
- Indexed
- SCIE
SCOPUS
- Journal Title
- International Journal of Nanomedicine
- Volume
- 10
- Start Page
- 115
- End Page
- 123
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/95975
- DOI
- 10.2147/IJN.S74924
- ISSN
- 1176-9114
- Abstract
- Nitric oxide (NO) is a messenger molecule involved in numerous physical and pathological processes in biological systems. Therefore, the development of a highly sensitive material able to detect NO in vivo is a key step in treating cardiovascular and a number of types of cancer-related diseases, as well as neurological dysfunction. Here we describe the development of a fluorescent probe using microbeads to enhance the fluorescence signal. Microbeads are infused with the fluorophore, dansyl-piperazine (Ds-pip), and quenched when the fluorophore is coordinated with a rhodium (Rh)-complex, ie, Rh2(AcO-)4(Ds-pip). In contrast, they are able to fluoresce when the transition-metal complex is replaced by NO. To confirm the “on/off” mechanism for detecting NO, we investigated the structural molecular properties using the Fritz Haber Institute ab initio molecular simulations (FHI-AIMS) package. According to the binding energy calculation, NO molecules bind more strongly and rapidly with the Rh-core of the Rh-complex than with Ds-pip. This suggests that NO can bond strongly with the Rh-core and replace Ds-pip, even though Ds-pip is already near the Rh-core. However, the recovery process takes longer than the quenching process because the recovery process needs to overcome the energy barrier for formation of the transition state complex, ie, NO-(AcO-)4-(Ds-pip). Further, we confirm that the Rh-complex with the Ds-pip structure has too small an energy gap to give off visible light from the highest unoccupied molecular orbital/lowest unoccupied molecular orbital energy level. © 2015 Yang et al.
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