Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

3,2 '-Dihydroxyflavone-Treated Pluripotent Stem Cells Show Enhanced Proliferation, Pluripotency Marker Expression, and Neuroprotective Properties

Authors
Han, DawoonKim, Han JunChoi, Hye YeonKim, BongWooYang, GwangmoHan, JihaeDayem, Ahmed AbdalLee, Hye-RimKim, Jin HoiLee, Kyung-MiJeong, Kyu-ShikDo, Sun HeeCho, Ssang-Goo
Issue Date
2015
Publisher
SAGE PUBLICATIONS INC
Keywords
3,2 ' -Dihydroxyflavone; Pluripotent stem cell; Self-renewal; Peripheral nerve injury model; Neuroprotective property
Citation
CELL TRANSPLANTATION, v.24, no.8, pp.1511 - 1532
Indexed
SCIE
SCOPUS
Journal Title
CELL TRANSPLANTATION
Volume
24
Number
8
Start Page
1511
End Page
1532
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/96123
DOI
10.3727/096368914X683511
ISSN
0963-6897
Abstract
Efficient maintenance of the undifferentiated status of embryonic stem cells (ESCs) may be important for preparation of high-quality cell sources that can be successfully used for stem cell research and therapy. Here we tried to identify a compound that can enhance the quality of pluripotent stem cells. Treatment of ESCs and induced pluripotent stem cells (iPSCs) with 3,2'-dihydroxyflavone (3,2'-DHF) led to increases in cell growth, colony formation, and cell proliferation. Treatment with 3,2'-DHF resulted in high expression of pluripotency markers (OCT4, SOX2, and NANOG) and significant activation (STAT3 and AKT) or suppression (GSK3 beta and ERK) of self-renewal-related kinases. 3,2'-DHF-treated high-quality pluripotent stem cells also showed enhanced differentiation potential. In particular, treatment of iPSCs with 3,2'-DHF led to elevated expression of ectodermal differentiation markers and improved differentiation into fully matured neurons. Next, we investigated the in vivo effect of 3,2'-DHF-pretreated iPSCs (3,2'-DHF iPSCs) in a peripheral nerve injury model and found that transplantation of 3,2'-DHF iPSCs resulted in more efficient axonal regeneration and functional recovery than in controls. Upon histopathological and gene expression analyses, we found that transplantation of 3,2'-DHF iPSCs stimulated expression of cytokines, such as TNF-alpha, in the early phase of injury and successfully reduced convalescence time of the injured peripheral nerve, showing an effective neuroprotective property. Taken together, our data suggest that 3,2'-DHF can be used for more efficient maintenance of pluripotent stem cells as well as for further applications in stem cell research and therapy.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biomedical Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Lee, Kyung Mi photo

Lee, Kyung Mi
의과학과
Read more

Altmetrics

Total Views & Downloads

BROWSE