A disposable bacterial lysis cartridge (BLC) suitable for an in situ water-borne pathogen detection system
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Eun-Hee | - |
dc.contributor.author | Lim, Hyun Jeong | - |
dc.contributor.author | Son, Ahjeong | - |
dc.contributor.author | Chua, Beelee | - |
dc.date.accessioned | 2021-09-05T00:55:35Z | - |
dc.date.available | 2021-09-05T00:55:35Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/96198 | - |
dc.description.abstract | We constructed a disposable bacterial lysis cartridge (BLC) suitable for an in situ pathogen detection system. It had an in-built micro corona discharge based ozone generator that provided ozone for cell lysis. Using a custom sample handling platform, its performance was evaluated with a Gram-positive bacterium of Bacillus subtilis. It was capable of achieving a similar degree of lysis as a commercial ultrasonic dismembrator with a P-1 microprobe in 10 min at an air pump flow rate of 29.4 ml min(-1) and an ozone generator operating voltage of 1600 V. The lysing duration could be significantly reduced to 5 min by increasing the air pump flow rate and the ozone generator operating voltage as well as by the addition of sodium dodecyl sulfate (SDS). | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | CORONA DISCHARGE | - |
dc.subject | OZONE PRODUCTION | - |
dc.subject | NANOGENE ASSAY | - |
dc.subject | RAPID METHOD | - |
dc.subject | DC CORONA | - |
dc.subject | DNA | - |
dc.subject | QUANTIFICATION | - |
dc.subject | RESISTANT | - |
dc.subject | SOIL | - |
dc.title | A disposable bacterial lysis cartridge (BLC) suitable for an in situ water-borne pathogen detection system | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chua, Beelee | - |
dc.identifier.doi | 10.1039/c5an01317h | - |
dc.identifier.scopusid | 2-s2.0-84946216978 | - |
dc.identifier.wosid | 000363677300038 | - |
dc.identifier.bibliographicCitation | ANALYST, v.140, no.22, pp.7776 - 7783 | - |
dc.relation.isPartOf | ANALYST | - |
dc.citation.title | ANALYST | - |
dc.citation.volume | 140 | - |
dc.citation.number | 22 | - |
dc.citation.startPage | 7776 | - |
dc.citation.endPage | 7783 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | CORONA DISCHARGE | - |
dc.subject.keywordPlus | OZONE PRODUCTION | - |
dc.subject.keywordPlus | NANOGENE ASSAY | - |
dc.subject.keywordPlus | RAPID METHOD | - |
dc.subject.keywordPlus | DC CORONA | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | RESISTANT | - |
dc.subject.keywordPlus | SOIL | - |
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