A disposable bacterial lysis cartridge (BLC) suitable for an in situ water-borne pathogen detection system
- Authors
- Lee, Eun-Hee; Lim, Hyun Jeong; Son, Ahjeong; Chua, Beelee
- Issue Date
- 2015
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- ANALYST, v.140, no.22, pp.7776 - 7783
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANALYST
- Volume
- 140
- Number
- 22
- Start Page
- 7776
- End Page
- 7783
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/96198
- DOI
- 10.1039/c5an01317h
- ISSN
- 0003-2654
- Abstract
- We constructed a disposable bacterial lysis cartridge (BLC) suitable for an in situ pathogen detection system. It had an in-built micro corona discharge based ozone generator that provided ozone for cell lysis. Using a custom sample handling platform, its performance was evaluated with a Gram-positive bacterium of Bacillus subtilis. It was capable of achieving a similar degree of lysis as a commercial ultrasonic dismembrator with a P-1 microprobe in 10 min at an air pump flow rate of 29.4 ml min(-1) and an ozone generator operating voltage of 1600 V. The lysing duration could be significantly reduced to 5 min by increasing the air pump flow rate and the ozone generator operating voltage as well as by the addition of sodium dodecyl sulfate (SDS).
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Collections - College of Engineering > School of Electrical Engineering > 1. Journal Articles
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