HtrA2/Omi influences the stability of LON protease 1 and prohibitin, proteins involved in mitochondrial homeostasis
- Authors
- Goo, Hui-Gwan; Rhim, Hyangshuk; Kang, Seongman
- Issue Date
- 1-11월-2014
- Publisher
- ELSEVIER INC
- Keywords
- HtrA2; Mitochondria; Mnd2; Protein quality control; PHB; Mitochondrial homeostasis
- Citation
- EXPERIMENTAL CELL RESEARCH, v.328, no.2, pp.456 - 465
- Indexed
- SCIE
SCOPUS
- Journal Title
- EXPERIMENTAL CELL RESEARCH
- Volume
- 328
- Number
- 2
- Start Page
- 456
- End Page
- 465
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/96812
- DOI
- 10.1016/j.yexcr.2014.07.032
- ISSN
- 0014-4827
- Abstract
- High temperature requirement A2 (HtrA2)/Omi is a serine protease localized in mitochondria. In response to apoptotic stimuli, HtrA2 is released to the cytoplasm and cleaves many proteins, including XIAP, Apollon/BRUCE, WT1, and Ped/Pea-15, to promote apoptosis. However, the function of HtrA2 in mitochondria under normal conditions remains unclear. Here, we show that the mitochondrial proteins, LON protease 1 (LONP1) and prohibitin (PHB), are overexpressed in HtrA2(-/-) mouse embryonic fibroblast (MEF) cells and HtrA2 knock-down HEK293T cells. We also confirm the effect of the HtrA2 protease on the stability of the above mitochondrial quality control proteins in motor neuron degeneration 2 (mnd2) mice, which have a greatly reduced protease activity as a result of a Ser276Cys missense mutation of the HtrA2 gene. In addition, PHB interacts with and is directly cleaved by HtrA2. Luminescence assays demonstrate that the intracellular ATP level is decreased in HtrA2(-/-) cells compared to HtrA2(+/+) cells. HtrA2 deficiency causes a decrease in the mitochondrial membrane potential, and reactive oxygen species (ROS) generation is greater in HtrA2(-/-) cells than in HtrA2(+/+) cells. Our results implicate that HtrA2 might be an upstream regulator of mitochondrial homeostasis. (C) 2014 Elsevier Inc. All rights reserved.
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