Rapid Detection of Mycobacterium tuberculosis Using a Novel Ultrafast Chip-Type Real-Time Polymerase Chain Reaction System
DC Field | Value | Language |
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dc.contributor.author | Lee, Seung Hyeun | - |
dc.contributor.author | Kim, Sung-Woo | - |
dc.contributor.author | Lee, Sehyun | - |
dc.contributor.author | Kim, EunSub | - |
dc.contributor.author | Kim, Duck-Joong | - |
dc.contributor.author | Park, Sohyun | - |
dc.contributor.author | Lee, Eun Joo | - |
dc.contributor.author | Lee, Sang Yeub | - |
dc.contributor.author | Lee, Ji Sung | - |
dc.contributor.author | Lim, Chae Seung | - |
dc.contributor.author | Kim, Won-Ki | - |
dc.contributor.author | In, Kwang Ho | - |
dc.date.accessioned | 2021-09-05T03:17:10Z | - |
dc.date.available | 2021-09-05T03:17:10Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-11 | - |
dc.identifier.issn | 0012-3692 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/96839 | - |
dc.description.abstract | BACKGROUND: NBS LabChip G2-3 is a novel, ultrafast, chip-type portable real-time polymerase chain reaction (PCR) system. We evaluated the clinical usefulness of this system in detecting pulmonary TB and assessed its diagnostic performance compared with a conventional tube-type PCR system. METHODS: A total of 247 sputum samples were collected from patients suspected of having pulmonary TB. Aft er the decontamination process, these samples were examined by fluorescence staining for acid-fast bacilli, cultures with both solid and liquid media, and real-time PCR with the NBS LabChip and a conventional tube-type system. The diagnostic accuracy of the NBS LabChip system and the agreement between the two assays were evaluated. RESULTS: Considering mycobacterial culture results as a gold standard, the overall sensitivity and specificity of the NBS LabChip was 83.8% (95% CI, 73.8%-91.1%) and 94.0% (95% CI, 89.3%-97.1%), respectively. For the detection of TB from the smear-positive samples, the sensitivity and specificity of the NBS LabChip was 96.0% (95% CI, 86.3%-99.5%) and 83.3% (95% CI, 72.3%-95.7%), respectively. For the smear-negative samples, the sensitivity and specificity of the NBS LabChip was 63.3% (95% CI, 43.9%-80.1%) and 95.0% (95% CI, 90.4%-97.8%), respectively. There were no significant differences in the sensitivity and specificity between the NBS LabChip and a conventional tube-type system, although the NBS LabChip shortened the PCR time (27 min for 45 cycles). CONCLUSIONS: The NBS LabChip G2-3 system has potential as an ultrafast, cost-effective diagnostic tool for pulmonary TB with high sensitivity and specificity. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | NUCLEIC-ACID AMPLIFICATION | - |
dc.subject | PULMONARY TUBERCULOSIS | - |
dc.subject | RESPIRATORY SPECIMENS | - |
dc.subject | DIAGNOSIS | - |
dc.subject | RESISTANCE | - |
dc.subject | COMPLEX | - |
dc.subject | TESTS | - |
dc.title | Rapid Detection of Mycobacterium tuberculosis Using a Novel Ultrafast Chip-Type Real-Time Polymerase Chain Reaction System | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Eun Joo | - |
dc.contributor.affiliatedAuthor | Lee, Sang Yeub | - |
dc.contributor.affiliatedAuthor | Lim, Chae Seung | - |
dc.contributor.affiliatedAuthor | Kim, Won-Ki | - |
dc.contributor.affiliatedAuthor | In, Kwang Ho | - |
dc.identifier.doi | 10.1378/chest.14-0626 | - |
dc.identifier.scopusid | 2-s2.0-84909990505 | - |
dc.identifier.wosid | 000347215500049 | - |
dc.identifier.bibliographicCitation | CHEST, v.146, no.5, pp.1319 - 1326 | - |
dc.relation.isPartOf | CHEST | - |
dc.citation.title | CHEST | - |
dc.citation.volume | 146 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1319 | - |
dc.citation.endPage | 1326 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | General & Internal Medicine | - |
dc.relation.journalResearchArea | Respiratory System | - |
dc.relation.journalWebOfScienceCategory | Critical Care Medicine | - |
dc.relation.journalWebOfScienceCategory | Respiratory System | - |
dc.subject.keywordPlus | NUCLEIC-ACID AMPLIFICATION | - |
dc.subject.keywordPlus | PULMONARY TUBERCULOSIS | - |
dc.subject.keywordPlus | RESPIRATORY SPECIMENS | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | TESTS | - |
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