Rapid Detection of Mycobacterium tuberculosis Using a Novel Ultrafast Chip-Type Real-Time Polymerase Chain Reaction System
- Authors
- Lee, Seung Hyeun; Kim, Sung-Woo; Lee, Sehyun; Kim, EunSub; Kim, Duck-Joong; Park, Sohyun; Lee, Eun Joo; Lee, Sang Yeub; Lee, Ji Sung; Lim, Chae Seung; Kim, Won-Ki; In, Kwang Ho
- Issue Date
- 11월-2014
- Publisher
- ELSEVIER SCIENCE BV
- Citation
- CHEST, v.146, no.5, pp.1319 - 1326
- Indexed
- SCIE
SCOPUS
- Journal Title
- CHEST
- Volume
- 146
- Number
- 5
- Start Page
- 1319
- End Page
- 1326
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/96839
- DOI
- 10.1378/chest.14-0626
- ISSN
- 0012-3692
- Abstract
- BACKGROUND: NBS LabChip G2-3 is a novel, ultrafast, chip-type portable real-time polymerase chain reaction (PCR) system. We evaluated the clinical usefulness of this system in detecting pulmonary TB and assessed its diagnostic performance compared with a conventional tube-type PCR system. METHODS: A total of 247 sputum samples were collected from patients suspected of having pulmonary TB. Aft er the decontamination process, these samples were examined by fluorescence staining for acid-fast bacilli, cultures with both solid and liquid media, and real-time PCR with the NBS LabChip and a conventional tube-type system. The diagnostic accuracy of the NBS LabChip system and the agreement between the two assays were evaluated. RESULTS: Considering mycobacterial culture results as a gold standard, the overall sensitivity and specificity of the NBS LabChip was 83.8% (95% CI, 73.8%-91.1%) and 94.0% (95% CI, 89.3%-97.1%), respectively. For the detection of TB from the smear-positive samples, the sensitivity and specificity of the NBS LabChip was 96.0% (95% CI, 86.3%-99.5%) and 83.3% (95% CI, 72.3%-95.7%), respectively. For the smear-negative samples, the sensitivity and specificity of the NBS LabChip was 63.3% (95% CI, 43.9%-80.1%) and 95.0% (95% CI, 90.4%-97.8%), respectively. There were no significant differences in the sensitivity and specificity between the NBS LabChip and a conventional tube-type system, although the NBS LabChip shortened the PCR time (27 min for 45 cycles). CONCLUSIONS: The NBS LabChip G2-3 system has potential as an ultrafast, cost-effective diagnostic tool for pulmonary TB with high sensitivity and specificity.
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- Appears in
Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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