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Efficacy of gaseous chlorine dioxide in inactivating Bacillus cereus spores attached to and in a biofilm on stainless steel

Authors
Nam, HyegyeongSeo, Hyun-SunBang, JihyunKim, HoikyungBeuchat, Larry R.Ryu, Jee-Hoon
Issue Date
1-Oct-2014
Publisher
ELSEVIER SCIENCE BV
Keywords
Gaseous chlorine dioxide; Bacillus cereus; Spores; Biofilm; Attachment; Stainless-steel surface
Citation
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, v.188, pp.122 - 127
Indexed
SCIE
SCOPUS
Journal Title
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume
188
Start Page
122
End Page
127
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/97127
DOI
10.1016/j.ijfoodmicro.2014.07.009
ISSN
0168-1605
Abstract
We evaluated the lethal activity of gaseous chlorine dioxide (ClO2) against Bacillus cereus spores attached to and in biofilm formed on a stainless steel surface. Aqueous ClO2 was prepared by mixing sulfuric acid (5% w/v) with sodium chlorite (10 ing/mL), and gaseous ClO2 was produced by vaporization of aqueous ClO2 in an air-tight container. The concentration of gaseous ClO2 in the air within the container increased rapidly at first but gradually decreased over time. The lethality of gaseous ClO2 against B. cereus spores attached to stainless steel coupons (SSCs) and in biofilm formed by the pathogen on SSCs was determined. The B. cereus spores attached to SSCs (53 0.1 log CPU/coupon) were completely inactivated within 1 h at 25 degrees C when treated with gaseous ClO2 (peak concentration: 1153 +/- 5.0 parts per million [ppm]). The total number of vegetative cells and spores in biofilm formed by B. cereus on SSCs was 5.9 +/- 0.3 log CPU/coupon; the spore count was 5.3 +/- 0.1 log CPU/coupon. The vegetative cells and spores in biofilm were completely inactivated within 6 h (peak concentration: 1153 +/- 5.0 ppm). Results show that B. cereus spores in biofilms are more resistant to gaseous ClO2 than are attached spores not in biofilrns. Gaseous ClO2 was, nevertheless, very effective in killing B. cereus spores in biofilm on the surface of stainless steel. Results show promise for application of gaseous ClO2 to enhance the microbiological safety of foods that may come in contact with stainless steel and possibly other hard surfaces on which B. cereus biofilms have formed. (C) 2014 Elsevier B.V. All rights reserved.
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