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ZnO nanorod matrix based electrochemical immunosensors for sensitivity enhanced detection of Legionella pneumophila

Authors
Park, JungilYou, XueqiuJang, YunseokNam, YoonkyungKim, Min JaMin, Nam KiPak, James Jungho
Issue Date
9월-2014
Publisher
ELSEVIER SCIENCE SA
Keywords
Zinc oxide; Nanorods; Electrochemistry; Immunosensor; Legionella pneumophila
Citation
SENSORS AND ACTUATORS B-CHEMICAL, v.200, pp.173 - 180
Indexed
SCIE
SCOPUS
Journal Title
SENSORS AND ACTUATORS B-CHEMICAL
Volume
200
Start Page
173
End Page
180
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/97530
DOI
10.1016/j.snb.2014.03.001
ISSN
0925-4005
Abstract
An ultrasensitive electrochemical immunosensor based on a ZnO nanorod (NR) matrix electrode was developed for detecting Legionella pneumophila for the first time. The ZnO NR matrix electrode was grown on an underlying Au working electrode (dia. 1.6 mm) using a hydrothermal method at a low temperature. A sandwich enzyme-linked immunosorbent assay was performed using the fabricated immunosensor. A primary antibody (1-4 mu g/mL) was immobilized onto the ZnO NR matrix electrode by electrostatic interaction and the peptidoglycan-associated lipoprotein of L. pneumophila, as a component of Legionella antigen (1-5000 pg/mL) was bound to the primary antibody. A secondary antibody (1-4 mu g/mL) conjugated to horseradish peroxidase was then bound to the antigen. The fabricated immunosensor was evaluated in a 0.1 M acetate buffer (pH 5) containing 0.05 mM 3,3',5,5'-tetramethylbenzidine and 1 mM H2O2 by cyclic voltammetry. In the fabricated immunosensors based on 1 mu g/mL and 2 mu g/mL antibody concentrations, the current output logarithmically increased with the L pneumophila antigen concentrations from 1 to 5000 pg/mL. The detection limit is similar to 1 pg/mL (S/N=4) with excellent selectivity. (C) 2014 Elsevier B.V. All rights reserved.
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