Microfluidic in-reservoir pre-concentration using a buffer drain technique
DC Field | Value | Language |
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dc.contributor.author | Yoon, Junghyo | - |
dc.contributor.author | Cho, Youngkyu | - |
dc.contributor.author | Han, Sewoon | - |
dc.contributor.author | Lim, Chae Seung | - |
dc.contributor.author | Lee, Jeong Hoon | - |
dc.contributor.author | Chung, Seok | - |
dc.date.accessioned | 2021-09-05T06:12:02Z | - |
dc.date.available | 2021-09-05T06:12:02Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-08-07 | - |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/97707 | - |
dc.description.abstract | Pre-concentration methods are essential for detecting low concentrations of influenza virus in biological samples from patients. Here, we describe a new method for draining buffer from solution in the reservoir of a microfluidic device to increase the concentration of virus in the reservoir. Viruses were captured in the reservoir by an ion depletion barrier from connected ion selective microfluidic channels. 75 mu l of buffer was successfully drained from a 100 mu l sample, resulting in a 4-fold increase in influenza hemagglutinin concentration in the reservoir. The volume of the final concentrated sample was suitable for detection of influenza hemagglutinin by the enzyme-linked immunosorbent assay, demonstrating the usefulness of the developed platform for enhanced sensitivity of virus detection in a conventional analysis. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | SAMPLE STACKING | - |
dc.title | Microfluidic in-reservoir pre-concentration using a buffer drain technique | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lim, Chae Seung | - |
dc.contributor.affiliatedAuthor | Chung, Seok | - |
dc.identifier.doi | 10.1039/c4lc00289j | - |
dc.identifier.scopusid | 2-s2.0-84903795028 | - |
dc.identifier.wosid | 000339306700020 | - |
dc.identifier.bibliographicCitation | LAB ON A CHIP, v.14, no.15, pp.2778 - 2782 | - |
dc.relation.isPartOf | LAB ON A CHIP | - |
dc.citation.title | LAB ON A CHIP | - |
dc.citation.volume | 14 | - |
dc.citation.number | 15 | - |
dc.citation.startPage | 2778 | - |
dc.citation.endPage | 2782 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.subject.keywordPlus | SAMPLE STACKING | - |
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