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Microfluidic in-reservoir pre-concentration using a buffer drain technique

Authors
Yoon, JunghyoCho, YoungkyuHan, SewoonLim, Chae SeungLee, Jeong HoonChung, Seok
Issue Date
7-8월-2014
Publisher
ROYAL SOC CHEMISTRY
Citation
LAB ON A CHIP, v.14, no.15, pp.2778 - 2782
Indexed
SCIE
SCOPUS
Journal Title
LAB ON A CHIP
Volume
14
Number
15
Start Page
2778
End Page
2782
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/97707
DOI
10.1039/c4lc00289j
ISSN
1473-0197
Abstract
Pre-concentration methods are essential for detecting low concentrations of influenza virus in biological samples from patients. Here, we describe a new method for draining buffer from solution in the reservoir of a microfluidic device to increase the concentration of virus in the reservoir. Viruses were captured in the reservoir by an ion depletion barrier from connected ion selective microfluidic channels. 75 mu l of buffer was successfully drained from a 100 mu l sample, resulting in a 4-fold increase in influenza hemagglutinin concentration in the reservoir. The volume of the final concentrated sample was suitable for detection of influenza hemagglutinin by the enzyme-linked immunosorbent assay, demonstrating the usefulness of the developed platform for enhanced sensitivity of virus detection in a conventional analysis.
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