Rapid Method for Determination of Anthocyanin Glucosides and Free Delphinidin in Grapes Using u-HPLC
- Authors
- Shim, You-Shin; Kim, Seunghee; Seo, Dongwon; Park, Hyun-Jin; Ha, Jaeho
- Issue Date
- 8월-2014
- Publisher
- OXFORD UNIV PRESS INC
- Citation
- JOURNAL OF CHROMATOGRAPHIC SCIENCE, v.52, no.7, pp.629 - 635
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CHROMATOGRAPHIC SCIENCE
- Volume
- 52
- Number
- 7
- Start Page
- 629
- End Page
- 635
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/97781
- DOI
- 10.1093/chromsci/bmt091
- ISSN
- 0021-9665
- Abstract
- A rapid method for the determination of anthocyanin glucosides and free delphinidin in food using ultra-high-performance liquid chromatography (u-HPLC) was validated through tests of precision, accuracy and linearity. The u-HPLC separation was performed on a reversed-phase C18 column (particle size 2 mu m, i.d. 2 mm, length 100 mm) with a photodiode array detector. The limits of detection and quantification of the u-HPLC analyses ranged from 0.07 to 0.65 mg/kg for nine types of anthocyanin glucosides and from 0.08 to 0.26 mg/kg for delphinidin aglycone. The intra-day and inter-day precision of individual anthocyanin glucosides and delphinidin aglycone were less than 9.42%. All calibration curves showed good linearity (coefficient of determination = 5 0.99) within the tested ranges. The rapid and simultaneous u-HPLC method presented in this study significantly improved the speed, sensitivity and resolution of the analyses of nine types of anthocyanin glucosides and free delphidinin aglycone in grapes.
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