Hempseed oil induces reactive oxygen species- and C/EBP homologous protein-mediated apoptosis in MH7A human rheumatoid arthritis fibroblast-like synovial cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong, Mini | - |
dc.contributor.author | Cho, Jaewook | - |
dc.contributor.author | Shin, Jong-Il | - |
dc.contributor.author | Jeon, Yong-Joon | - |
dc.contributor.author | Kim, Jin-Hyun | - |
dc.contributor.author | Lee, Sung-Joon | - |
dc.contributor.author | Kim, Eun-Soo | - |
dc.contributor.author | Lee, Kyungho | - |
dc.date.accessioned | 2021-09-05T07:06:49Z | - |
dc.date.available | 2021-09-05T07:06:49Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-07-03 | - |
dc.identifier.issn | 0378-8741 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/97980 | - |
dc.description.abstract | Ethnopharmacological relevance: The medicinal efficacy of hempseed (Cannabis sativa L.), which is rich in polyunsaturated fatty acids, in atopic dermatitis, inflammation, and rheumatoid arthritis (RA) has been suggested for centuries. Hempseed has been used as a treatment for these diseases in Korean and Chinese folk medicine. The aim of the study is to investigate the effects of hempseed oil (HO) on MH7A human RA fibroblast-like synovial cells. Materials and methods: MH7A cells were used to study the anti-rheumatoid effects of hempseed (Cannabis sativa L., cv. Cheungsam/Cannabaceae) oil by investigating cell viability, apoptosis, lipid accumulation, oxidative stress, and endoplasmic reticulum (ER) stress-induced apoptosis. Results: HO treatment reduced the survival rate of MH7A cells and promoted apoptotic cell death in a time- and dose-dependent manner. Both lipid accumulation and the level of intracellular reactive oxygen species (ROS) increased in HO-treated MH7A cells. Co-treatment with the antioxidant Tiron effectively abrogated the cytotoxic effects of HO; the ROS level was reduced, cell viability was recovered, and apoptotic cell death was significantly diminished. Moreover, HO-treated cells exhibited increased expression of the major ER stress markers, glucose-regulated protein 78 and C/EBP homologous protein (CHOP). The siRNA-mediated knockdown of CHOP prevented HO-induced apoptosis. Conclusions: Our results suggest that HO treatment induced lipid accumulation, ROS production, CHOP expression, and apoptosis in MH7A cells, and that CHOP functions as an anti-rheumatoid factor downstream of HO in MH7A cells. (C) 2014 Elsevier Ireland Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.subject | ENDOPLASMIC-RETICULUM STRESS | - |
dc.subject | GAMMA-LINOLENIC ACID | - |
dc.subject | CHOP | - |
dc.subject | GROWTH | - |
dc.subject | OMEGA-3-FATTY-ACIDS | - |
dc.subject | PROLIFERATION | - |
dc.subject | PATHOGENESIS | - |
dc.subject | INFLAMMATION | - |
dc.subject | CHOLESTEROL | - |
dc.subject | EXPRESSION | - |
dc.title | Hempseed oil induces reactive oxygen species- and C/EBP homologous protein-mediated apoptosis in MH7A human rheumatoid arthritis fibroblast-like synovial cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Sung-Joon | - |
dc.identifier.doi | 10.1016/j.jep.2014.04.052 | - |
dc.identifier.scopusid | 2-s2.0-84902553522 | - |
dc.identifier.wosid | 000339035900022 | - |
dc.identifier.bibliographicCitation | JOURNAL OF ETHNOPHARMACOLOGY, v.154, no.3, pp.745 - 752 | - |
dc.relation.isPartOf | JOURNAL OF ETHNOPHARMACOLOGY | - |
dc.citation.title | JOURNAL OF ETHNOPHARMACOLOGY | - |
dc.citation.volume | 154 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 745 | - |
dc.citation.endPage | 752 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM STRESS | - |
dc.subject.keywordPlus | GAMMA-LINOLENIC ACID | - |
dc.subject.keywordPlus | CHOP | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | OMEGA-3-FATTY-ACIDS | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | PATHOGENESIS | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | CHOLESTEROL | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | Hempseed oil | - |
dc.subject.keywordAuthor | Rheumatoid arthritis | - |
dc.subject.keywordAuthor | Polyunsaturated fatty acids | - |
dc.subject.keywordAuthor | Synovial cell | - |
dc.subject.keywordAuthor | ER stress | - |
dc.subject.keywordAuthor | Apoptosis | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.