Semi-Quantitative Analyses of Hippocampal Heat Shock Protein-70 Expression Based on the Duration of lschemia and the Volume of Cerebral Infarction in Mice
DC Field | Value | Language |
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dc.contributor.author | Choi, Jong-Il | - |
dc.contributor.author | Kim, Sang-Dae | - |
dc.contributor.author | Kim, Se-Hoon | - |
dc.contributor.author | Lim, Dong-Jun | - |
dc.contributor.author | Ha, Sung-Kon | - |
dc.date.accessioned | 2021-09-05T08:26:20Z | - |
dc.date.available | 2021-09-05T08:26:20Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-06 | - |
dc.identifier.issn | 2005-3711 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/98389 | - |
dc.description.abstract | Objective : We investigated the expression of hippocampal heat shock protein 70 (HSP-70) infarction volume after different durations of experimental ischemic stroke in mice. Methods : Focal cerebral ischemia was induced in mice by occluding the middle cerebral artery with the modified intraluminal filament technique. Twenty-four hours after ischemia induction, both hippocampi were extracted for HSP-70 protein analyses. Slices from each hemisphere were stained with 2,3,5-triphenyltetrazolium chloride (2%), and infarction volumes were calculated. HSP-70 levels were evaluated using western blot and enzyme-linked immunosorbent assay (ELISA). HSP-70 subtype (hsp70.1, hspa1a, hspa1b) mRNA levels in the hippocampus were measured using reverse transcription-polymerase chain reaction (RT-PCR). Results : Cerebral infarctions were found ipsilateral to the occlusion in 10 mice exposed to transient ischemia (5 each in the 30-min and 60-min occlusion groups), whereas no focal infarctions were noted in any of the sham mice. The average infarct volumes of the 2 ischemic groups were 22.28 +/- 7.31 mm(3) [30-min group standard deviation (SD)] and 38.06 +/- 9.53 mm(3) (60-min group SD). Western blot analyses and ELISA showed that HSP-70 in hippocampal tissues increased in the infarction groups than in the sham group. However, differences in HSP-70 levels between the 2 infarction groups were statistically insignificant. Moreover, RT-PCR results demonstrated no relationship between the mRNA expression of HSP-70 subtypes and occlusion time or infarction volume. Conclusion : Our results indicated no significant difference in HSP-70 expression between the 30- and 60-min occlusion groups despite the statistical difference in infarction volumes. Furthermore, HSP-70 subtype mRNA expression was independent of both occlusion duration and cerebral infarction volume. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN NEUROSURGICAL SOC | - |
dc.subject | HEAT-SHOCK PROTEINS | - |
dc.subject | DIFFERENTIAL EXPRESSION | - |
dc.subject | MOLECULAR CHAPERONES | - |
dc.subject | ARTERY OCCLUSION | - |
dc.subject | BRAIN ISCHEMIA | - |
dc.subject | RATS | - |
dc.subject | PROTECTION | - |
dc.subject | HSP70 | - |
dc.subject | GENE | - |
dc.subject | NEUROPROTECTION | - |
dc.title | Semi-Quantitative Analyses of Hippocampal Heat Shock Protein-70 Expression Based on the Duration of lschemia and the Volume of Cerebral Infarction in Mice | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Sang-Dae | - |
dc.contributor.affiliatedAuthor | Kim, Se-Hoon | - |
dc.contributor.affiliatedAuthor | Lim, Dong-Jun | - |
dc.contributor.affiliatedAuthor | Ha, Sung-Kon | - |
dc.identifier.doi | 10.3340/jkns.2014.55.6.307 | - |
dc.identifier.scopusid | 2-s2.0-84929919158 | - |
dc.identifier.wosid | 000341228800001 | - |
dc.identifier.bibliographicCitation | JOURNAL OF KOREAN NEUROSURGICAL SOCIETY, v.55, no.6, pp.307 - 312 | - |
dc.relation.isPartOf | JOURNAL OF KOREAN NEUROSURGICAL SOCIETY | - |
dc.citation.title | JOURNAL OF KOREAN NEUROSURGICAL SOCIETY | - |
dc.citation.volume | 55 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 307 | - |
dc.citation.endPage | 312 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalWebOfScienceCategory | Clinical Neurology | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.subject.keywordPlus | HEAT-SHOCK PROTEINS | - |
dc.subject.keywordPlus | DIFFERENTIAL EXPRESSION | - |
dc.subject.keywordPlus | MOLECULAR CHAPERONES | - |
dc.subject.keywordPlus | ARTERY OCCLUSION | - |
dc.subject.keywordPlus | BRAIN ISCHEMIA | - |
dc.subject.keywordPlus | RATS | - |
dc.subject.keywordPlus | PROTECTION | - |
dc.subject.keywordPlus | HSP70 | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | NEUROPROTECTION | - |
dc.subject.keywordAuthor | Cerebral infarction volume | - |
dc.subject.keywordAuthor | Heat shock protein | - |
dc.subject.keywordAuthor | Occlusion time | - |
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