Semi-Quantitative Analyses of Hippocampal Heat Shock Protein-70 Expression Based on the Duration of lschemia and the Volume of Cerebral Infarction in Mice
- Authors
- Choi, Jong-Il; Kim, Sang-Dae; Kim, Se-Hoon; Lim, Dong-Jun; Ha, Sung-Kon
- Issue Date
- 6월-2014
- Publisher
- KOREAN NEUROSURGICAL SOC
- Keywords
- Cerebral infarction volume; Heat shock protein; Occlusion time
- Citation
- JOURNAL OF KOREAN NEUROSURGICAL SOCIETY, v.55, no.6, pp.307 - 312
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF KOREAN NEUROSURGICAL SOCIETY
- Volume
- 55
- Number
- 6
- Start Page
- 307
- End Page
- 312
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/98389
- DOI
- 10.3340/jkns.2014.55.6.307
- ISSN
- 2005-3711
- Abstract
- Objective : We investigated the expression of hippocampal heat shock protein 70 (HSP-70) infarction volume after different durations of experimental ischemic stroke in mice. Methods : Focal cerebral ischemia was induced in mice by occluding the middle cerebral artery with the modified intraluminal filament technique. Twenty-four hours after ischemia induction, both hippocampi were extracted for HSP-70 protein analyses. Slices from each hemisphere were stained with 2,3,5-triphenyltetrazolium chloride (2%), and infarction volumes were calculated. HSP-70 levels were evaluated using western blot and enzyme-linked immunosorbent assay (ELISA). HSP-70 subtype (hsp70.1, hspa1a, hspa1b) mRNA levels in the hippocampus were measured using reverse transcription-polymerase chain reaction (RT-PCR). Results : Cerebral infarctions were found ipsilateral to the occlusion in 10 mice exposed to transient ischemia (5 each in the 30-min and 60-min occlusion groups), whereas no focal infarctions were noted in any of the sham mice. The average infarct volumes of the 2 ischemic groups were 22.28 +/- 7.31 mm(3) [30-min group standard deviation (SD)] and 38.06 +/- 9.53 mm(3) (60-min group SD). Western blot analyses and ELISA showed that HSP-70 in hippocampal tissues increased in the infarction groups than in the sham group. However, differences in HSP-70 levels between the 2 infarction groups were statistically insignificant. Moreover, RT-PCR results demonstrated no relationship between the mRNA expression of HSP-70 subtypes and occlusion time or infarction volume. Conclusion : Our results indicated no significant difference in HSP-70 expression between the 30- and 60-min occlusion groups despite the statistical difference in infarction volumes. Furthermore, HSP-70 subtype mRNA expression was independent of both occlusion duration and cerebral infarction volume.
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