The Effect of Alendronate-Loaded Polycarprolactone Nanofibrous Scaffolds on Osteogenic Differentiation of Adipose-Derived Stem Cells in Bone Tissue Regeneration
- Authors
- Yun, Young-Pil; Kim, Seung-Ju; Lim, Youn-Mook; Park, Kyeongsoon; Kim, Hak-Jun; Jeong, Sung-In; Kim, Sung Eun; Song, Hae-Ryong
- Issue Date
- 6월-2014
- Publisher
- AMER SCIENTIFIC PUBLISHERS
- Keywords
- Polycarprolactone; Alendronate; Nanofiber; Adipose-Derived Stem Cells; Bone Regeneration
- Citation
- JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, v.10, no.6, pp.1080 - 1090
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
- Volume
- 10
- Number
- 6
- Start Page
- 1080
- End Page
- 1090
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/98390
- DOI
- 10.1166/jbn.2014.1819
- ISSN
- 1550-7033
- Abstract
- The osteogenic effect of culturing adipose-derived stem cells (ADSCs) on alendronate (Aln)-loaded polycarprolactone (PCL) nanofibrous scaffolds was evaluated by examining alkaline phosphatase (ALP) activity, calcium content, and expression of osteogenic differentiation genes in vitro. The 10% Aln/PCL nanofibrous scaffolds showed more ALP activity, mineralization, and osteocalcin and osteopontin mRNA than the 1% or 5% Aln/PCL nanofibrous scaffolds. The capacity of Aln/PCL nanofibrous scaffolds to regenerate new bone was studied in a rat calvarial defect model. New bone formation in vivo was evaluated by radiography, micro-computed tomography, and histological analysis. At 8 weeks after implantation, Aln/PCL scaffolds had a positive effect on bone regeneration and matrix formation. These results suggested that Aln/PCL nanofibrous scaffolds enhanced the osteogenic differentiation of ADSCs in vitro and bone formation in vivo.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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