Optimization of pancreatic lipase inhibition by Cudrania tricuspidata fruits using response surface methodology
- Authors
- Jeong, Ji Yeon; Jo, Yang Hee; Lee, Ki Yong; Do, Seon-Gil; Hwang, Bang Yeon; Lee, Mi Kyeong
- Issue Date
- 15-5월-2014
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Cudrania tricuspidata; Response surface methodology; Box-Behnken design; Pancreatic lipase; 5,7,4 ' -Trihydroxy-6,8-diprenylisoflavone; Optimization
- Citation
- BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, v.24, no.10, pp.2329 - 2333
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
- Volume
- 24
- Number
- 10
- Start Page
- 2329
- End Page
- 2333
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/98506
- DOI
- 10.1016/j.bmcl.2014.03.067
- ISSN
- 0960-894X
- Abstract
- The fruits of Cudrania tricuspidata (Carr.) Bur. (Moraceae) significantly inhibited pancreatic lipase, which plays a key role in fat absorption. Optimization of extraction conditions with minimum pancreatic lipase activity and maximum yield was determined using response surface methodology with three-level-three-factor Box-Behnken design (BBD). Regression analysis showed a good fit of the experimental data and the optimal condition was obtained as ethanol concentration, 74.5%; temperature 61.9 degrees C and extraction time, 13.5 h. The pancreatic lipase activity and extraction yield under optimal conditions were found to be 65.5% and 54.0%, respectively, which were well matched with the predicted value of 65.8% and 47.1%. Further fractionation of C. tricuspidata extract resulted in the isolation of compound 1, which was identified as 5,7,4'-trihydroxy-6,8-diprenylisoflavone. It inhibited pancreatic lipase activity with IC50 value of 65.0 mu M. HPLC analysis suggested positive correlation between pancreatic lipase inhibition and 5,7,4 '-trihydroxy-6,8-diprenylisoflavone of C. tricuspidata fruits. (C) 2014 Elsevier Ltd. All rights reserved.
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