Destabilization of i-Motif by Submolar Concentrations of a Monovalent Cation
DC Field | Value | Language |
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dc.contributor.author | Kim, Sung Eun | - |
dc.contributor.author | Lee, Il-Buem | - |
dc.contributor.author | Hyeon, Changbong | - |
dc.contributor.author | Hong, Seok-Cheol | - |
dc.date.accessioned | 2021-09-05T08:54:12Z | - |
dc.date.available | 2021-09-05T08:54:12Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-05-08 | - |
dc.identifier.issn | 1520-6106 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/98526 | - |
dc.description.abstract | Counterions are crucial for self-assembly of nucleic acids. Submolar monovalent cations are generally deemed to stabilize various types of base pairs in nucleic acids such as Watson-Crick and Hoogsteen base pairs via screening of electrostatic repulsion. Besides monovalent cations, acidic pH is required for i-motif formation because protons facilitate pairing between cytosines. Here we report that Li+ ions destabilize i-motif, whereas other monovalent cations, Na+ and K+, have the usual stabilizing effect. The thermodynamics data alone, however, cannot reveal which mechanism, enhanced unfolding or suppressed folding or both, is responsible for the Li+-induced destabilization. To gain further insight, we examined the kinetics of i-motif. To deal with slow kinetics of i-motif, we developed a method dubbed HaRP to construct a long FRET time trace to observe a sufficient number of transitions. Our kinetics analysis shows clearly that Li+ ions promote unfolding of i-motif but do not hinder its folding, lending strong support for our hypothesis on the origin of this unusual effect of Li+. Although the subangstrom size of Li+ ions allows them to infiltrate the space between cytosines in competition with protons, they cannot adequately fulfill the role of protons in mediating the hydrogen bonding of cytosine pairs. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | RICH STRAND | - |
dc.subject | DNA NANOMACHINE | - |
dc.subject | HUMAN TELOMERE | - |
dc.subject | BASE-PAIRS | - |
dc.subject | PH CHANGES | - |
dc.subject | STABILITY | - |
dc.subject | RNA | - |
dc.subject | KINETICS | - |
dc.subject | FRAGMENT | - |
dc.subject | PROTEIN | - |
dc.title | Destabilization of i-Motif by Submolar Concentrations of a Monovalent Cation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hong, Seok-Cheol | - |
dc.identifier.doi | 10.1021/jp500120d | - |
dc.identifier.scopusid | 2-s2.0-84900326986 | - |
dc.identifier.wosid | 000335878000004 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PHYSICAL CHEMISTRY B, v.118, no.18, pp.4753 - 4760 | - |
dc.relation.isPartOf | JOURNAL OF PHYSICAL CHEMISTRY B | - |
dc.citation.title | JOURNAL OF PHYSICAL CHEMISTRY B | - |
dc.citation.volume | 118 | - |
dc.citation.number | 18 | - |
dc.citation.startPage | 4753 | - |
dc.citation.endPage | 4760 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.subject.keywordPlus | RICH STRAND | - |
dc.subject.keywordPlus | DNA NANOMACHINE | - |
dc.subject.keywordPlus | HUMAN TELOMERE | - |
dc.subject.keywordPlus | BASE-PAIRS | - |
dc.subject.keywordPlus | PH CHANGES | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | KINETICS | - |
dc.subject.keywordPlus | FRAGMENT | - |
dc.subject.keywordPlus | PROTEIN | - |
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