Rhus verniciflua Extract Modulates Survival of MCF-7 Breast Cancer Cells through the Modulation of AMPK-Pathway
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jung Ok | - |
dc.contributor.author | Moon, Ji Wook | - |
dc.contributor.author | Lee, Soo Kyung | - |
dc.contributor.author | Kim, So Mi | - |
dc.contributor.author | Kim, Nami | - |
dc.contributor.author | Ko, Seong-Gyu | - |
dc.contributor.author | Kim, Hyeon Soo | - |
dc.contributor.author | Park, Sun Hwa | - |
dc.date.accessioned | 2021-09-05T09:00:35Z | - |
dc.date.available | 2021-09-05T09:00:35Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-05 | - |
dc.identifier.issn | 0918-6158 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/98568 | - |
dc.description.abstract | Rhus verniciflua STOKES (RVS) is used as an anti-cancer agent in traditional herbal medicine. However, the underlying molecular mechanism of its action is poorly understood. Here, we elucidated the mechanism of the anti-cancer mechanism of RVS in MCF-7 human breast cancer cells. We found that RVS increased the phosphorylation of AMP-activated protein kinase (AMPK) and downstream acetyl-CoA carboxylase (ACC) and suppressed cell viability in an AMPK-dependent fashion. RVS also induced an increase in reactive oxygen species (ROS) levels. RVS-induced AMPK phosphorylation was not observed in the presence of N-acetyl-cysteine (NAC), which indicated that ROS is associated with RVS-induced AMPK phosphorylation. In addition, fluorescent staining (Annexin V/propidium iodide) revealed that RVS increased the expression of Annexin V, which indicates that RVS leads to cancer-induced apoptosis. Moreover, RVS increased the phosphorylation of p53 and the expression of Bax. The inhibition of AMPK blocked RVS-induced p53 phosphorylation and Bax expression, which suggests that AMPK is involved in RVS-induced cancer apoptosis. Taken together, these results demonstrate that RVS has anti-tumor effects on MCF-7 cells through an AMPK-signaling pathway. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PHARMACEUTICAL SOC JAPAN | - |
dc.subject | ACTIVATED PROTEIN-KINASE | - |
dc.subject | N-TERMINAL KINASE | - |
dc.subject | MITOCHONDRIAL-MEMBRANE | - |
dc.subject | METABOLIC CHECKPOINT | - |
dc.subject | INDUCE APOPTOSIS | - |
dc.subject | ENERGY STATUS | - |
dc.subject | KEY SENSOR | - |
dc.subject | STOKES | - |
dc.subject | GROWTH | - |
dc.subject | BAX | - |
dc.title | Rhus verniciflua Extract Modulates Survival of MCF-7 Breast Cancer Cells through the Modulation of AMPK-Pathway | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Hyeon Soo | - |
dc.contributor.affiliatedAuthor | Park, Sun Hwa | - |
dc.identifier.doi | 10.1248/bpb.b13-00893 | - |
dc.identifier.scopusid | 2-s2.0-84899890449 | - |
dc.identifier.wosid | 000335273700011 | - |
dc.identifier.bibliographicCitation | BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.37, no.5, pp.794 - 801 | - |
dc.relation.isPartOf | BIOLOGICAL & PHARMACEUTICAL BULLETIN | - |
dc.citation.title | BIOLOGICAL & PHARMACEUTICAL BULLETIN | - |
dc.citation.volume | 37 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 794 | - |
dc.citation.endPage | 801 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | N-TERMINAL KINASE | - |
dc.subject.keywordPlus | MITOCHONDRIAL-MEMBRANE | - |
dc.subject.keywordPlus | METABOLIC CHECKPOINT | - |
dc.subject.keywordPlus | INDUCE APOPTOSIS | - |
dc.subject.keywordPlus | ENERGY STATUS | - |
dc.subject.keywordPlus | KEY SENSOR | - |
dc.subject.keywordPlus | STOKES | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | BAX | - |
dc.subject.keywordAuthor | Rhus verniciflua | - |
dc.subject.keywordAuthor | AMP-activated protein kinase (AMPK) | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | MCF-7 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.