Rhus verniciflua Extract Modulates Survival of MCF-7 Breast Cancer Cells through the Modulation of AMPK-Pathway
- Authors
- Lee, Jung Ok; Moon, Ji Wook; Lee, Soo Kyung; Kim, So Mi; Kim, Nami; Ko, Seong-Gyu; Kim, Hyeon Soo; Park, Sun Hwa
- Issue Date
- 5월-2014
- Publisher
- PHARMACEUTICAL SOC JAPAN
- Keywords
- Rhus verniciflua; AMP-activated protein kinase (AMPK); apoptosis; MCF-7
- Citation
- BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.37, no.5, pp.794 - 801
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOLOGICAL & PHARMACEUTICAL BULLETIN
- Volume
- 37
- Number
- 5
- Start Page
- 794
- End Page
- 801
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/98568
- DOI
- 10.1248/bpb.b13-00893
- ISSN
- 0918-6158
- Abstract
- Rhus verniciflua STOKES (RVS) is used as an anti-cancer agent in traditional herbal medicine. However, the underlying molecular mechanism of its action is poorly understood. Here, we elucidated the mechanism of the anti-cancer mechanism of RVS in MCF-7 human breast cancer cells. We found that RVS increased the phosphorylation of AMP-activated protein kinase (AMPK) and downstream acetyl-CoA carboxylase (ACC) and suppressed cell viability in an AMPK-dependent fashion. RVS also induced an increase in reactive oxygen species (ROS) levels. RVS-induced AMPK phosphorylation was not observed in the presence of N-acetyl-cysteine (NAC), which indicated that ROS is associated with RVS-induced AMPK phosphorylation. In addition, fluorescent staining (Annexin V/propidium iodide) revealed that RVS increased the expression of Annexin V, which indicates that RVS leads to cancer-induced apoptosis. Moreover, RVS increased the phosphorylation of p53 and the expression of Bax. The inhibition of AMPK blocked RVS-induced p53 phosphorylation and Bax expression, which suggests that AMPK is involved in RVS-induced cancer apoptosis. Taken together, these results demonstrate that RVS has anti-tumor effects on MCF-7 cells through an AMPK-signaling pathway.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
- College of Medicine > Department of Medical Science > 1. Journal Articles
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