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A red-shifted fluorescent substrate for aldehyde dehydrogenase
- Authors
- Minn, Il; Wang, Haofan; Mease, Ronnie C.; Byun, Youngjoo; Yang, Xing; Wang, Julia; Leach, Steven D.; Pomper, Martin G.
- Issue Date
- 4월-2014
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- NATURE COMMUNICATIONS, v.5
- Indexed
- SCIE
SCOPUS
- Journal Title
- NATURE COMMUNICATIONS
- Volume
- 5
- ISSN
- 2041-1723
- Abstract
- Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a greenfluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDH(pos) cells. We demonstrate that AldeRed 588-A successfully isolates ALDH(hi) human haematopoietic stem cells from heterogeneous cord blood mononuclear cells. AldeRed 588-A can be used for multicolour applications to fractionate ALDH(pos) cells in the presence of green fluorophores including the ALDEFLUOR reagent and cells expressing enhanced green-fluorescent protein (eGFP). AldeRed 588-A stains ALDHpos murine pancreatic centroacinar and terminal duct cells, as visualized using fluorescent microscopy. AldeRed588-A provides a useful tool to select stem cells or study ALDH within a green-fluorescent background.
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Collections - College of Pharmacy > Department of Pharmaceutical Science > 1. Journal Articles
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