Identification of the cis-element and bZIP DNA binding motifs for the autogenous negative control of mouse NOSTRIN
- Authors
- Bae, Seong-Ho; Choi, Young-Joon; Kim, Kyung-hyun; Park, Sung-Soo
- Issue Date
- 17-1월-2014
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- NOSTRIN; bZIP; Inverted-repeat
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.443, no.3, pp.924 - 931
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 443
- Number
- 3
- Start Page
- 924
- End Page
- 931
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/99529
- DOI
- 10.1016/j.bbrc.2013.12.066
- ISSN
- 0006-291X
- Abstract
- mNOSTRIN is the mouse ortholog of hNOSTRIN. Unlike hNOSTRIN, which is alternatively spliced to produce two isoforms (alpha and beta), only a single isoform of mNOSTRIN has been detected in either the nucleus or cytoplasm/membrane. Because mNOSTRIN represses its own transcription through direct binding onto its own promoter, this protein is constantly expressed in a temporally regulated pattern during differentiation of F9 embryonic carcinoma cells. In this study, we identified the specific cis-element in the mNOSTRIN regulatory region that is responsible for negative autogenous control. This element exhibits inverted dyad symmetry. Furthermore, we identified a putative bZIP motif in the middle region of mNOSTRIN, which is responsible for DNA binding, and showed that disruption of the leucine zippers abolished the DNA-binding activity of mNOSTRIN. Here, we report that a single form of mNOSTRIN functions in both the nucleus and cytoplasm/membrane. In the nucleus, mNOSTRIN acts as a transcriptional repressor by binding to the cis-element through its bZIP motif. (C) 2013 Elsevier Inc. All rights reserved.
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Collections - Graduate School > Department of Biotechnology and Bioinformatics > 1. Journal Articles
- College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
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