An ultra-sensitive detection of a whole virus using dual aptamers developed by immobilization-free screening
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Jee-Woong | - |
dc.contributor.author | Lee, Su Jin | - |
dc.contributor.author | Choi, Eun-Jin | - |
dc.contributor.author | Kim, Jaejo | - |
dc.contributor.author | Song, Jae-Young | - |
dc.contributor.author | Gu, Man Bock | - |
dc.date.accessioned | 2021-09-05T12:17:54Z | - |
dc.date.available | 2021-09-05T12:17:54Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014-01-15 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/99536 | - |
dc.description.abstract | In this study, we successfully developed a ssDNA aptamer pairs by using an advanced immobilization-free SELEX method with affinity-based selection and counter-screening process at every round. By implementing this method, two different aptamers specifically binding to bovine viral diarrhea virus type 1(BVDV type 1) with high affinity were successfully screened. This aptamer pair was applied to ultrasensitive detection platform for BVDV type 1 in a sandwich manner. The ultrasensitive detection of BVDV type 1 using one of aptamers conjugated with gold nanoparticles was obtained in aptamer-aptamer sandwich type sensing format, with the limit of detection of 800 copies/ml, which is comparable to a real-time PCR method. (C) 2013 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.subject | VIRAL-DIARRHEA-VIRUS | - |
dc.subject | POLYMERASE-CHAIN-REACTION | - |
dc.subject | RT-PCR | - |
dc.subject | SIGNAL ENHANCEMENT | - |
dc.subject | CAPILLARY-ELECTROPHORESIS | - |
dc.subject | GRAPHENE | - |
dc.subject | CATTLE | - |
dc.subject | INFECTIONS | - |
dc.subject | SELECTION | - |
dc.subject | IMMUNOHISTOCHEMISTRY | - |
dc.title | An ultra-sensitive detection of a whole virus using dual aptamers developed by immobilization-free screening | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Gu, Man Bock | - |
dc.identifier.doi | 10.1016/j.bios.2013.07.052 | - |
dc.identifier.scopusid | 2-s2.0-84883307628 | - |
dc.identifier.wosid | 000326905500049 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.51, pp.324 - 329 | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 51 | - |
dc.citation.startPage | 324 | - |
dc.citation.endPage | 329 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | VIRAL-DIARRHEA-VIRUS | - |
dc.subject.keywordPlus | POLYMERASE-CHAIN-REACTION | - |
dc.subject.keywordPlus | RT-PCR | - |
dc.subject.keywordPlus | SIGNAL ENHANCEMENT | - |
dc.subject.keywordPlus | CAPILLARY-ELECTROPHORESIS | - |
dc.subject.keywordPlus | GRAPHENE | - |
dc.subject.keywordPlus | CATTLE | - |
dc.subject.keywordPlus | INFECTIONS | - |
dc.subject.keywordPlus | SELECTION | - |
dc.subject.keywordPlus | IMMUNOHISTOCHEMISTRY | - |
dc.subject.keywordAuthor | Dual aptamers | - |
dc.subject.keywordAuthor | Bovine viral diarrhea virus (BVDV) | - |
dc.subject.keywordAuthor | SELEX | - |
dc.subject.keywordAuthor | SPR | - |
dc.subject.keywordAuthor | Sandwich | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.