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An ultra-sensitive detection of a whole virus using dual aptamers developed by immobilization-free screening

Authors
Park, Jee-WoongLee, Su JinChoi, Eun-JinKim, JaejoSong, Jae-YoungGu, Man Bock
Issue Date
15-1월-2014
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Keywords
Dual aptamers; Bovine viral diarrhea virus (BVDV); SELEX; SPR; Sandwich
Citation
BIOSENSORS & BIOELECTRONICS, v.51, pp.324 - 329
Indexed
SCIE
SCOPUS
Journal Title
BIOSENSORS & BIOELECTRONICS
Volume
51
Start Page
324
End Page
329
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/99536
DOI
10.1016/j.bios.2013.07.052
ISSN
0956-5663
Abstract
In this study, we successfully developed a ssDNA aptamer pairs by using an advanced immobilization-free SELEX method with affinity-based selection and counter-screening process at every round. By implementing this method, two different aptamers specifically binding to bovine viral diarrhea virus type 1(BVDV type 1) with high affinity were successfully screened. This aptamer pair was applied to ultrasensitive detection platform for BVDV type 1 in a sandwich manner. The ultrasensitive detection of BVDV type 1 using one of aptamers conjugated with gold nanoparticles was obtained in aptamer-aptamer sandwich type sensing format, with the limit of detection of 800 copies/ml, which is comparable to a real-time PCR method. (C) 2013 Elsevier B.V. All rights reserved.
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