Comparison of ELISA and HPLC methods for the determination of biogenic amines in commercial doenjang and gochujang

초록

In order to conduct fast detection of biogenic amines (BAs) in doenjang and gochujang, a competitive direct enzyme-linked immunosorbent assay (CD-ELISA) for histamine was compared with HPLC analysis. The histamine contents were determined from 0.7 to 420 mg/kg by CD-ELISA and 2.2 to 382.4 mg/kg by HPLC. Both methods showed a good agreement for histamine analysis (R=0.969). In addition, the histamine contents by CD-ELISA were correlated to the sum of 7 major BAs in the samples (R=0.910), suggesting that the CD-ELISA can be used as a rapid indicator for total BAs in doenjang and gochujang.

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