항균제 및 항진균제 첨가를 통한 효모 및 유산균의 선택배지 제조

초록

In this study, we examined whether the of yeast mold agar and de Man, Rogosa and Sharpe (MRS) agar was improved when antibacterial and antifungal agents were added. The addition of 0.01 g/L of chloramphenicol, a widely used antibacterial agent, was sufficient to inhibit the growth of lactic acid bacteria (LAB), and the addition 0.5 g/L of the antifungal agent, cycloheximide, inhibited yeast growth. The antifungal agents, amphotericin B and methyl paraben, poorly inhibited yeast growth at concentrations of 0.5 and 0.28 g/L, respectively. Moreover, it was shown that the addition of chloramphenicol or cycloheximide selectively inhibited the growth of LAB or yeast in the makgeolli sample, whereby both yeast and LAB were present. These results suggest that these selective media can be used to count in various types of samples in which LAB and yeast coexist.

키워드