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A highly sensitive immunoassay using antibody-conjugated spherical mesoporous silica with immobilized enzymes
- Authors
- Eum, Ji Young; Hwang, Sang Youn; Ju, Youngjun; Shim, Jong Min; Piao, Yunxian; Lee, Jinwoo; Kim, Hak-Sung; Kim, Jungbae
- Issue Date
- 2014
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- CHEMICAL COMMUNICATIONS, v.50, no.27, pp.3546 - 3548
- Indexed
- SCIE
SCOPUS
- Journal Title
- CHEMICAL COMMUNICATIONS
- Volume
- 50
- Number
- 27
- Start Page
- 3546
- End Page
- 3548
- ISSN
- 1359-7345
- Abstract
- A highly sensitive immunoassay was developed by using antibody-conjugated spherical mesoporous silica with immobilized enzymes. The higher ratio of enzyme/antibody than conventional ELISA improved both the sensitivity and dynamic range. Especially, the use of spherical mesoporous silica could achieve a limit of detection (LOD) with a sensitivity that is 20 times more than that of ELISA using amorphous silica.
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Related Researcher
- Kim, Jung bae
- College of Engineering (Department of Chemical and Biological Engineering)