Anti-inflammatory effects of anethole in lipopolysaccharide-induced acute lung injury in mice
- Authors
- Kang, Purum; Kim, Ka Young; Lee, Hui Su; Min, Sun Seek; Seol, Geun Hee
- Issue Date
- 5-12월-2013
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Anethole; LPS; TNF-alpha; Acute lung injury
- Citation
- LIFE SCIENCES, v.93, no.24, pp.955 - 961
- Indexed
- SCIE
SCOPUS
- Journal Title
- LIFE SCIENCES
- Volume
- 93
- Number
- 24
- Start Page
- 955
- End Page
- 961
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/101313
- DOI
- 10.1016/j.lfs.2013.10.014
- ISSN
- 0024-3205
- Abstract
- Aims: Anethole, the major component of the essential oil of star anise, has been reported to have antioxidant, antibacterial, antifungal, anti-inflammatory, and anesthetic properties. In this study, we investigated the anti-inflammatory effects of anethole in a mouse model of acute lung injury induced by lipopolysaccharide (LPS). Main methods: BALB/C mice were intraperitoneally administered anethole (62.5, 125, 250, or 500 mg/kg) 1 h before intratracheal treatment with LPS (1.5 mg/kg) and sacrificed after 4 h. The anti-inflammatory effects of anethole were assessed by measuring total protein and cell levels and inflammatory mediator production and by histological evaluation and Western blot analysis. Key findings: LPS significantly increased total protein levels; numbers of total cells, including macrophages and neutrophils; and the production of inflammatory mediators such as matrix metalloproteinase 9 (MMP-9), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and nitric oxide (NO) in bronchoalveolar lavage fluid. Anethole (250 mg/kg) decreased total protein concentrations; numbers of inflammatory cells, including neutrophils and macrophages; and the inflammatory mediators MMP-9, TNE-alpha and NO. In addition, pretreatment with anethole decreased LPS-induced histopathological changes. The anti-inflammatory mechanism of anethole in LPS-induced acute lung injury was assessed by investigating the effects of anethole on NF-kappa B activation. Anethole suppressed the activation of NF-kappa B by blocking I kappa B-alpha degradation. Significance: These results, showing that anethole prevents LPS-induced acute lung inflammation in mice, suggest that anethole may be therapeutically effective in inflammatory conditions in humans. (C) 2013 Elsevier Inc. All rights reserved.
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