Expression, crystallization and preliminary X-ray crystallographic studies of SCP3 coiled-coil domain

Abstract

The synaptonemal complex protein SCP3 is one of the components of the lateral element of the synaptonemal complex, which is a meiosis-specific complex structure formed at the synapse of homologous chromosomes. In this study, a C-terminal coiled-coil domain, SCP3, was overexpressed in Escherichia coli with an engineered C-terminal His tag. The coiled-coil domain of SCP3 was then purified to homogeneity and crystallized at 293 K. X-ray diffraction data were collected to a resolution of 3.2 angstrom from a crystal belonging to space group C2, with unit-cell parameters a = 121.29, b = 43.08, c = 57.42 angstrom, alpha = 100.71 degrees. The asymmetric unit was estimated to contain three molecules.