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Amplification of Resonant Rayleigh Light Scattering Response Using Immunogold Colloids for Detection of Lysozyme

Authors
Phuoc Long TruongChoi, Seung PhillSim, Sang Jun
Issue Date
25-10월-2013
Publisher
WILEY-V C H VERLAG GMBH
Keywords
LSPR; rayleigh scattering; immunogold colloids; plasmon coupling; aptasensors
Citation
SMALL, v.9, no.20, pp.3485 - 3492
Indexed
SCIE
SCOPUS
Journal Title
SMALL
Volume
9
Number
20
Start Page
3485
End Page
3492
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/101846
DOI
10.1002/smll.201202638
ISSN
1613-6810
Abstract
A strategy for attomolar-level detection of small molecule-size proteins is reported based on Rayleigh light scattering spectroscopy of individual nanoplasmonic aptasensors by exploiting the outstanding characteristics of gold colloids to amplify the nontransparent resonant signal at ultralow analyte concentrations. The fabrication method utilizes thiol-mediated adsorption of a DNA aptamer on the immobilized Au nanoparticle surface, the interfacial binding characteristics of the aptamer with its target molecules, and the antibody-antigen interaction through plasmonic resonance coupling of the Au nanoparticles. Using lysozyme as a model analyte for disease detection, the detection limit of the aptasensor is approximate to 7 x 10(3) aM, corresponding to the LSPR (max) shift of approximate to 2.25 nm. Up to a 380% increase in the localized resonant (max) shift is demonstrated upon antibody binding to the analyte compared to the primary response during signal amplification using immunogold colloids. This enhancement leads to a limit of detection of approximate to 7 aM, which is an improvement of three orders of magnitude. The results demonstrate substantial promise for developing coupled plasmonic nanostructures for ultrasensitive detection of various biological and chemical analytes.
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공과대학 (화공생명공학과)
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