A simultaneous analysis method for Caihu-Dayuan-Yin using reversed-phase high-performance liquid chromatography coupled with pulsed amperometric detection
- Authors
- Kwon, Ha-Jeong; Kim, Ji-Hwan; Park, Yong-Duk; Hong, Seon-Pyo
- Issue Date
- 5월-2013
- Publisher
- PHARMACEUTICAL SOC KOREA
- Keywords
- Reversed-phased high performance liquid chromatography; Pulsed amperometric detection; Caihu-Dayuan-Yin; Sensitivity
- Citation
- ARCHIVES OF PHARMACAL RESEARCH, v.36, no.5, pp.579 - 585
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- ARCHIVES OF PHARMACAL RESEARCH
- Volume
- 36
- Number
- 5
- Start Page
- 579
- End Page
- 585
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/103333
- DOI
- 10.1007/s12272-013-0040-7
- ISSN
- 0253-6269
- Abstract
- A simultaneous analysis method was developed for Caihu-Dayuan-Yin decoction containing Bupleuri Radix, Glycyrrhizae Radix, and Ponciri fructus by reversed-phase (RP) HPLC coupled with pulsed amperometric detection (PAD). The analytes were separated in a RP column with 0.01 % phosphoric acid-acetonitrile gradient elution. The limits of detection (S/N = 3) and of quantification (S/N = 10) ranged 0.1-3.0, and 0.3-10 ng, respectively. The intra- and interday precisions (RSDs) were a parts per thousand currency sign15.15 % and the average recoveries were 94.6-98.3 %. Our RP-HPLC-PAD method showed 1.7-100 times higher sensitivity than the RP-HPLC-UV method.
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