Modeling and optimization of lipase-catalyzed esterification of policosanols with conjugated linoleic acid by response surface methodology
DC Field | Value | Language |
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dc.contributor.author | Zhao, Tingting | - |
dc.contributor.author | Ha, Tae-Yeoul | - |
dc.contributor.author | Lee, Junsoo | - |
dc.contributor.author | Kim, Byung Hee | - |
dc.contributor.author | Kim, In-Hwan | - |
dc.date.accessioned | 2021-09-06T03:13:32Z | - |
dc.date.available | 2021-09-06T03:13:32Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-04 | - |
dc.identifier.issn | 1024-2422 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/103679 | - |
dc.description.abstract | The aim of this study was to model the lipase-catalyzed esterification of policosanols with conjugated linoleic acid (CLA) in a solvent-free system to produce wax esters which had a lower melting point than that of their corresponding policosanol forms and to optimize the reaction conditions by response surface methodology (RSM). Novozym 435 was selected as a suitable biocatalyst for the reaction. The molar ratio of substrates (policosanols to CLA) was 1:2. A well-fitting quadratic polynomial regression model for the degree of esterification (DE) of policosanols with CLA was established with regard to temperature (35-65 degrees C), enzyme loading (1-5% of weight of total substrates), and reaction time (10-50 min). Optimal reaction conditions were 61.3 degrees C for temperature, 3.7% for enzyme loading, and 34.1 min for reaction time, and the DE was similar to 95 mol% under these conditions. The policosanols and wax esters synthesized under optimal conditions had melting points of 79 degrees C and 57 degrees C, respectively. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | INFORMA HEALTHCARE | - |
dc.subject | WAX ESTERS | - |
dc.subject | RATS | - |
dc.subject | ABSORPTION | - |
dc.subject | OIL | - |
dc.subject | FAT | - |
dc.title | Modeling and optimization of lipase-catalyzed esterification of policosanols with conjugated linoleic acid by response surface methodology | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, In-Hwan | - |
dc.identifier.doi | 10.3109/10242422.2013.777434 | - |
dc.identifier.scopusid | 2-s2.0-84876354362 | - |
dc.identifier.wosid | 000317939100006 | - |
dc.identifier.bibliographicCitation | BIOCATALYSIS AND BIOTRANSFORMATION, v.31, no.2, pp.114 - 122 | - |
dc.relation.isPartOf | BIOCATALYSIS AND BIOTRANSFORMATION | - |
dc.citation.title | BIOCATALYSIS AND BIOTRANSFORMATION | - |
dc.citation.volume | 31 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 114 | - |
dc.citation.endPage | 122 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | WAX ESTERS | - |
dc.subject.keywordPlus | RATS | - |
dc.subject.keywordPlus | ABSORPTION | - |
dc.subject.keywordPlus | OIL | - |
dc.subject.keywordPlus | FAT | - |
dc.subject.keywordAuthor | Conjugated linoleic acid | - |
dc.subject.keywordAuthor | Novozym 435 | - |
dc.subject.keywordAuthor | policosanols | - |
dc.subject.keywordAuthor | response surface methodology | - |
dc.subject.keywordAuthor | wax ester | - |
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