Modeling and optimization of lipase-catalyzed esterification of policosanols with conjugated linoleic acid by response surface methodology
- Authors
- Zhao, Tingting; Ha, Tae-Yeoul; Lee, Junsoo; Kim, Byung Hee; Kim, In-Hwan
- Issue Date
- 4월-2013
- Publisher
- INFORMA HEALTHCARE
- Keywords
- Conjugated linoleic acid; Novozym 435; policosanols; response surface methodology; wax ester
- Citation
- BIOCATALYSIS AND BIOTRANSFORMATION, v.31, no.2, pp.114 - 122
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCATALYSIS AND BIOTRANSFORMATION
- Volume
- 31
- Number
- 2
- Start Page
- 114
- End Page
- 122
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/103679
- DOI
- 10.3109/10242422.2013.777434
- ISSN
- 1024-2422
- Abstract
- The aim of this study was to model the lipase-catalyzed esterification of policosanols with conjugated linoleic acid (CLA) in a solvent-free system to produce wax esters which had a lower melting point than that of their corresponding policosanol forms and to optimize the reaction conditions by response surface methodology (RSM). Novozym 435 was selected as a suitable biocatalyst for the reaction. The molar ratio of substrates (policosanols to CLA) was 1:2. A well-fitting quadratic polynomial regression model for the degree of esterification (DE) of policosanols with CLA was established with regard to temperature (35-65 degrees C), enzyme loading (1-5% of weight of total substrates), and reaction time (10-50 min). Optimal reaction conditions were 61.3 degrees C for temperature, 3.7% for enzyme loading, and 34.1 min for reaction time, and the DE was similar to 95 mol% under these conditions. The policosanols and wax esters synthesized under optimal conditions had melting points of 79 degrees C and 57 degrees C, respectively.
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Collections - College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles
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