CD44-negative cells in head and neck squamous carcinoma also have stem-cell like traits
- Authors
- Oh, Se-Yeong; Kang, Hyun Jung; Kim, Young Sook; Kim, Hyunggee; Lim, Young Chang
- Issue Date
- 1월-2013
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Neoplastic stem cell; Head and neck neoplasm; Biomarkers; CD44
- Citation
- EUROPEAN JOURNAL OF CANCER, v.49, no.1, pp.272 - 280
- Indexed
- SCIE
SCOPUS
- Journal Title
- EUROPEAN JOURNAL OF CANCER
- Volume
- 49
- Number
- 1
- Start Page
- 272
- End Page
- 280
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/104415
- DOI
- 10.1016/j.ejca.2012.06.004
- ISSN
- 0959-8049
- Abstract
- CD44 is generally accepted as a surrogate marker for head and neck squamous carcinoma cancer stem cells (HNSC CSCs) and only CD44+ HNSC cells have tumour initiating capacity. However, a recent report suggested that CSCs themselves might be heterogenous due to various genetic alterations. Here, we compared in vitro stem-like cell characteristics, chemoresistance and in vivo tumour formation capacity of CD44+ and CD44- HNSC cells obtained from primary HNSC patient specimens. CD44- HNSC spheroid cells generated spheroid cells again after seeding of single-dissociated spheroid CD44- HNSC cells. Immunocytochemistry assays revealed that various stem cell markers, including octamer-binding transcription factor 4 (OCT4), sex determining region Y-box 2 (SOX2) and nestin were up-regulated in CD44- spheroid cells, similar to CD44+ spheroid cells. Furthermore, CD44- spheroid cells appeared to be chemoresistant to cisplatin and showed increased levels of ABCG2, similar to CD44+ spheroid cells. Of most interest, as few as 1000 CD44- spheroid cells were able to give rise to tumours in nude mice. The collective data indicate that the cell surface marker CD44 cannot be used as a selective marker of spheroid-forming, tumour-initiating or chemoresistant cell populations, and further indicate the limitation of current HNSC CSC identification methods using the CD44 cell surface marker. (C) 2012 Elsevier Ltd. All rights reserved.
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