Functional 3D Human Primary Hepatocyte Spheroids Made by Co-Culturing Hepatocytes from Partial Hepatectomy Specimens and Human Adipose-Derived Stem Cells
- Authors
- No, Da Yoon; Lee, Seung-A; Choi, Yoon Young; Park, DoYeun; Jang, Ju Yun; Kim, Dong-Sik; Lee, Sang-Hoon
- Issue Date
- 7-12월-2012
- Publisher
- PUBLIC LIBRARY SCIENCE
- Citation
- PLOS ONE, v.7, no.12
- Indexed
- SCIE
SCOPUS
- Journal Title
- PLOS ONE
- Volume
- 7
- Number
- 12
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/106669
- DOI
- 10.1371/journal.pone.0050723
- ISSN
- 1932-6203
- Abstract
- We have generated human hepatocyte spheroids with uniform size and shape by co-culturing 1:1 mixtures of primary human hepatocytes (hHeps) from partial hepatectomy specimens and human adipose-derived stem cells (hADSCs) in concave microwells. The hADSCs in spheroids could compensate for the low viability and improve the functional maintenance of hHeps. Co-cultured spheroids aggregated and formed compact spheroidal shapes more rapidly, and with a significantly higher viability than mono-cultured spheroids. The liver-specific functions of co-cultured spheroids were greater, although they contained half the number of hepatocytes as mono-cultured spheroids. Albumin secretion by co-cultured spheroids was 10% higher on day 7, whereas urea secretion was similar, compared with mono-cultured spheroids. A quantitative cytochrome P450 assay showed that the enzymatic activity of co-cultured spheroids cultured for 9 days was 28% higher than that of mono-cultured spheroids. These effects may be due to the transdifferentiation potential and paracrine healing effects of hADSCs on hHeps. These co-cultured spheroids may be useful for creating artificial three-dimensional hepatic tissue constructs and for cell therapy with limited numbers of human hepatocytes. Citation: No DY, Lee S-A, Choi YY, Park D, Jang JY, et al. (2012) Functional 3D Human Primary Hepatocyte Spheroids Made by Co-Culturing Hepatocytes from Partial Hepatectomy Specimens and Human Adipose-Derived Stem Cells. PLoS ONE 7(12): e50723. doi:10.1371/journal.pone.0050723
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Collections - College of Health Sciences > School of Biomedical Engineering > 1. Journal Articles
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