Proteomic profiling of tumor-initiating cells in HT-29 human colorectal cancer cells
- Authors
- Lee, Han-Na; Park, Soo-Hyun; Lee, Eun-Kyung; Bernardo, Raymundo; Kim, Chan-Wha
- Issue Date
- 12-10월-2012
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Cancer stem cell; Colorectal cancer; CD133; Two dimensional gel electrophoresis; Proteomics
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.427, no.1, pp.171 - 177
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 427
- Number
- 1
- Start Page
- 171
- End Page
- 177
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/107213
- DOI
- 10.1016/j.bbrc.2012.09.036
- ISSN
- 0006-291X
- Abstract
- Recent reports have suggested that tumors are organized in heterogeneous populations. Within these populations, a small subpopulation of cells is more capable of initiating malignancy; these are called cancer stem cells. In this study, HT-29 cells were sorted according to the presence or absence of the cancer stem cell marker CD133. We confirmed that CD133+ cells possessed higher clonogenicity compared to CD133- cells. Furthermore, proteomic analysis identified 10 proteins, including actin-related protein 2/3 complex subunit 5-like and profilin 2. In conclusion, our data demonstrated that the expression of specific proteins associated with metastasis and invasion in CD133+ cells contributed to the sternness and tumorigenic properties of these cells. (C) 2012 Elsevier Inc. All rights reserved.
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Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
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