Comparison of tear proteins between healthy and early diabetic retinopathy patients
- Authors
- Kim, Hyun-Jung; Kim, Pan-Kyeom; Yoo, Hyun-Syuk; Kim, Chan-Wha
- Issue Date
- 1월-2012
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Diabetic retinopathy; Nonproliferative diabetic retinopathy; Proliferative diabetic retinopathy; Tear protein; Two-dimensional gel electrophoresis
- Citation
- CLINICAL BIOCHEMISTRY, v.45, no.1-2, pp.60 - 67
- Indexed
- SCIE
SCOPUS
- Journal Title
- CLINICAL BIOCHEMISTRY
- Volume
- 45
- Number
- 1-2
- Start Page
- 60
- End Page
- 67
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/109158
- DOI
- 10.1016/j.clinbiochem.2011.10.006
- ISSN
- 0009-9120
- Abstract
- Objectives: To identify potential prognostic or diagnostic marker tear proteins for early diabetic retinopathy (DR) and to investigate the pathogenesis of this disease using proteomics techniques. Design and methods: The tear proteins expressed in patients suffering from diabetes mellitus without the retinopathy symptoms, nonproliferative diabetic retinopathy and healthy volunteers were analyzed by 2-DE. The differentially expressed proteins in patients were identified by ESI-Q-TOF and confirmed by Western blotting Results: Proteins which were differentially expressed with statistical significance (P < 0.05) in two diabetic groups as compared to those in healthy group were selected and identified by ESI-Q-TOF MS/MS. Among these proteins, three proteins (LCN-1, HSP27 and B2M) were found to exhibit a progressive reduction in two disease groups. The expression levels of which might be useful as diagnostic biomarkers of DR were verified by Western blotting Conclusions: Proteomic analysis using tear is a novel approach that can provide insight into possible biomarker and the pathogenesis of early DR. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
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