Use of immobilized phospholipase A(1)catalyzed acidolysis for the production of structured phosphatidylcholine with an elevated conjugated linoleic acid content
- Authors
- Baeza-Jimenez, R.; Gonzalez-Rodriguez, J.; Kim, In-H.; Garcia, H. S.; Otero, C.
- Issue Date
- 1월-2012
- Publisher
- CONSEJO SUPERIOR INVESTIGACIONES CIENTIFICAS-CSIC
- Keywords
- Acidolysis; Conjugated linoleic acid; Phosphatidylcholine; Phospholipase A(1); Structured phospholipids
- Citation
- GRASAS Y ACEITES, v.63, no.1, pp.44 - 52
- Indexed
- SCIE
SCOPUS
- Journal Title
- GRASAS Y ACEITES
- Volume
- 63
- Number
- 1
- Start Page
- 44
- End Page
- 52
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/109215
- DOI
- 10.3989/gya.045211
- ISSN
- 0017-3495
- Abstract
- Structured phosphatidylcholine (SPC) was successfully produced via immobilized phospholipase A(1) (PLA(1)) - catalyzed acidolysis of phosphatidylcholine (PC) with conjugated linoleic acid (CLA). The effects of enzyme loading (2, 5, 10, 15 and 20%, with respect to the weight of substrates), temperature (20, 30, 40, 50 and 60 degrees C) and the molar ratio of substrates (1:2, 1:4, 1:6, 1:8 and 1:10, PC/CLA) were evaluated to maximize the incorporation of CLA into PC. The maximum incorporation of CLA achieved was ca. 90% for 24 h of reaction at 50 degrees C and 200 rpm, using a 1:4 substrate molar ratio and an enzyme loading of 15%.
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Collections - College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles
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