Use of immobilized phospholipase A(1)catalyzed acidolysis for the production of structured phosphatidylcholine with an elevated conjugated linoleic acid content

Abstract

Structured phosphatidylcholine (SPC) was successfully produced via immobilized phospholipase A(1) (PLA(1)) - catalyzed acidolysis of phosphatidylcholine (PC) with conjugated linoleic acid (CLA). The effects of enzyme loading (2, 5, 10, 15 and 20%, with respect to the weight of substrates), temperature (20, 30, 40, 50 and 60 degrees C) and the molar ratio of substrates (1:2, 1:4, 1:6, 1:8 and 1:10, PC/CLA) were evaluated to maximize the incorporation of CLA into PC. The maximum incorporation of CLA achieved was ca. 90% for 24 h of reaction at 50 degrees C and 200 rpm, using a 1:4 substrate molar ratio and an enzyme loading of 15%.