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A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay

Authors
Lee, Hye-JinKim, Byoung ChanOh, Min-KyuKim, Jungbae
Issue Date
2012
Publisher
ROYAL SOC CHEMISTRY
Citation
CHEMICAL COMMUNICATIONS, v.48, no.48, pp.5971 - 5973
Indexed
SCIE
SCOPUS
Journal Title
CHEMICAL COMMUNICATIONS
Volume
48
Number
48
Start Page
5971
End Page
5973
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/109289
DOI
10.1039/c2cc30710c
ISSN
1359-7345
Abstract
Stable enzyme precipitate coatings (EPCs) with high enzyme loading were employed to develop a sensitive and reliable detection protocol, termed EPC-linked aptamer assay (EPC-LAA). EPC-LAA achieved the limit of detection to be 0.5 ng per mL of thrombin (13.5 pM), and maintained a reasonably stable detection signal even after storing all the reagents at 40 degrees C for 66 days.
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Kim, Jung bae
공과대학 (화공생명공학과)
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