Silk protein hydrolysate increases glucose uptake through up-regulation of GLUT 4 and reduces the expression of leptin in 3T3-L1 fibroblast
DC Field | Value | Language |
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dc.contributor.author | Lee, Hyun-Sun | - |
dc.contributor.author | Lee, Hyun Jung | - |
dc.contributor.author | Suh, Hyung Joo | - |
dc.date.accessioned | 2021-09-07T05:31:17Z | - |
dc.date.available | 2021-09-07T05:31:17Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2011-12 | - |
dc.identifier.issn | 0271-5317 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/110994 | - |
dc.description.abstract | The purpose of our research was to test the hypothesis that silk protein hydrolysate increases glucose uptake in cultured murine embryonic fibroblasts. Insulin sensitizing activity was observed in a cell-based glucose uptake assay using 3T3-L1 embryonic fibroblasts. The treatment of 1 mg/mL of silk peptide E5K6 plus 0.2 nM insulin was associated with a significant increase in glucose uptake (124.0% +/- 2.5%) compared to treatment with 0.2 nM insulin alone. When the 3T3-L1 cells were induced to differentiate into fibroblasts, fat droplets formed inside the cells. Silk peptide E5K6 reduced the formation of fat droplets at the 1-mg/mL dosage (86.1% +/- 2.5%) when compared to the control (100.0% +/- 5.8%). A 1 mg/mL dose of silk peptide E5K6 significantly increased GLUT 4 expression (131.5% +/- 4.0%). The treatment of 1 mg/mL of silk peptide E5K6 did not present any changes for adipogenic expressed genes, but leptin expression was significantly increased by silk peptide E5K6 supplementation (175.9% +/- 11.1%). From these results, silk peptide E5K6 increased glucose uptake via up-regulation of GLUT 4 and decreased fat accumulation via the up-regulation of leptin. (C) 2011 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.subject | ADIPOCYTE DIFFERENTIATION | - |
dc.subject | BOMBYX-MORI | - |
dc.subject | METABOLISM | - |
dc.subject | FIBROIN | - |
dc.subject | CELLS | - |
dc.subject | ADIPOGENESIS | - |
dc.subject | GROWTH | - |
dc.subject | GAMMA | - |
dc.subject | MICE | - |
dc.title | Silk protein hydrolysate increases glucose uptake through up-regulation of GLUT 4 and reduces the expression of leptin in 3T3-L1 fibroblast | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Hyun Jung | - |
dc.contributor.affiliatedAuthor | Suh, Hyung Joo | - |
dc.identifier.doi | 10.1016/j.nutres.2011.09.009 | - |
dc.identifier.scopusid | 2-s2.0-83355169560 | - |
dc.identifier.wosid | 000298517600009 | - |
dc.identifier.bibliographicCitation | NUTRITION RESEARCH, v.31, no.12, pp.937 - 943 | - |
dc.relation.isPartOf | NUTRITION RESEARCH | - |
dc.citation.title | NUTRITION RESEARCH | - |
dc.citation.volume | 31 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 937 | - |
dc.citation.endPage | 943 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Nutrition & Dietetics | - |
dc.relation.journalWebOfScienceCategory | Nutrition & Dietetics | - |
dc.subject.keywordPlus | ADIPOCYTE DIFFERENTIATION | - |
dc.subject.keywordPlus | BOMBYX-MORI | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | FIBROIN | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | ADIPOGENESIS | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | GAMMA | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordAuthor | Silk protein hydrolysate | - |
dc.subject.keywordAuthor | 3T3-L1 fibroblasts | - |
dc.subject.keywordAuthor | Insulin-stimulated glucose uptake | - |
dc.subject.keywordAuthor | Adipogenesis | - |
dc.subject.keywordAuthor | Leptin | - |
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