Silk protein hydrolysate increases glucose uptake through up-regulation of GLUT 4 and reduces the expression of leptin in 3T3-L1 fibroblast
- Authors
- Lee, Hyun-Sun; Lee, Hyun Jung; Suh, Hyung Joo
- Issue Date
- 12월-2011
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Silk protein hydrolysate; 3T3-L1 fibroblasts; Insulin-stimulated glucose uptake; Adipogenesis; Leptin
- Citation
- NUTRITION RESEARCH, v.31, no.12, pp.937 - 943
- Indexed
- SCIE
SCOPUS
- Journal Title
- NUTRITION RESEARCH
- Volume
- 31
- Number
- 12
- Start Page
- 937
- End Page
- 943
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/110994
- DOI
- 10.1016/j.nutres.2011.09.009
- ISSN
- 0271-5317
- Abstract
- The purpose of our research was to test the hypothesis that silk protein hydrolysate increases glucose uptake in cultured murine embryonic fibroblasts. Insulin sensitizing activity was observed in a cell-based glucose uptake assay using 3T3-L1 embryonic fibroblasts. The treatment of 1 mg/mL of silk peptide E5K6 plus 0.2 nM insulin was associated with a significant increase in glucose uptake (124.0% +/- 2.5%) compared to treatment with 0.2 nM insulin alone. When the 3T3-L1 cells were induced to differentiate into fibroblasts, fat droplets formed inside the cells. Silk peptide E5K6 reduced the formation of fat droplets at the 1-mg/mL dosage (86.1% +/- 2.5%) when compared to the control (100.0% +/- 5.8%). A 1 mg/mL dose of silk peptide E5K6 significantly increased GLUT 4 expression (131.5% +/- 4.0%). The treatment of 1 mg/mL of silk peptide E5K6 did not present any changes for adipogenic expressed genes, but leptin expression was significantly increased by silk peptide E5K6 supplementation (175.9% +/- 11.1%). From these results, silk peptide E5K6 increased glucose uptake via up-regulation of GLUT 4 and decreased fat accumulation via the up-regulation of leptin. (C) 2011 Elsevier Inc. All rights reserved.
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