c-Fos regulates hepatitis C virus propagation
- Authors
- Kang, Sang-Min; Lim, Seri; Won, Seung-Jae; Shin, Ye-Jin; Lim, Yun-Sook; Ahn, Byung-Yoon; Hwang, Soon B.
- Issue Date
- 20-Oct-2011
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Hepatitis C virus; c-Fos; Cellular factor; Propagation; Pathogenesis
- Citation
- FEBS LETTERS, v.585, no.20, pp.3236 - 3244
- Indexed
- SCIE
SCOPUS
- Journal Title
- FEBS LETTERS
- Volume
- 585
- Number
- 20
- Start Page
- 3236
- End Page
- 3244
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/111358
- DOI
- 10.1016/j.febslet.2011.08.041
- ISSN
- 0014-5793
- Abstract
- Hepatitis C virus (HCV) RNA replication requires cellular factors as well as viral non-structural proteins (NS protein). Using small interfering RNA (siRNA) library screening, we previously identified c-Fos as a host factor involved in HCV propagation. In the present study, we demonstrated that silencing of c-Fos expression resulted in decrease of HCV propagation in cell culture grown HCV (HCVcc)-infected cells; whereas overexpression of c-Fos significantly increased HCV propagation. We further confirmed the positive role of c-Fos in HCV propagation by both HCV-luciferase reporter assay and immunofluorescence analysis. We showed that c-Fos level was upregulated by HCV infection. Furthermore, phorbol 12-myristate 13-acetate (PMA)-induced c-Fos level was synergistically increased by HCV infection. These data suggest that c-Fos acts as a positive regulator of HCV propagation and may contribute to HCV-associated pathogenesis. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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