A novel alkaline lipase from Ralstonia with potential application in biodiesel production
- Authors
- Yoo, Hah-Young; Simkhada, Jaya Ram; Cho, Seung Sik; Park, Don Hee; Kim, Seung Wook; Seong, Chi Nam; Yoo, Jin Cheol
- Issue Date
- 5월-2011
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Ralstonia sp CS274; Lipase; Purification; Biodiesel production
- Citation
- BIORESOURCE TECHNOLOGY, v.102, no.10, pp.6104 - 6111
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 102
- Number
- 10
- Start Page
- 6104
- End Page
- 6111
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/112614
- DOI
- 10.1016/j.biortech.2011.02.046
- ISSN
- 0960-8524
- Abstract
- With the aim of isolating a biocatalyst able to catalyze biodiesel production from microbial source, Ralstonia sp. CS274 was isolated and a lipase from the strain (RL74) was purified. Molecular weight of RL74 was estimated to be 28,000 Da by SDS-PAGE. The activity was highest at 50-55 degrees C and pH 8.0-9.5 and was stable at pH 7.0-12.0 and up to 45 degrees C. It was resistant to oxidizing and reducing agents and the activity was enhanced by detergents. RL74 was 1,3 specific and K-m and V-max for p-nitrophenyl palmitate were 2.73 +/- 0.6 mM and 101.4 +/- 1.9 mM/min mg, respectively. N-terminal amino acid sequence showed partial homology with that of Penicillium lipases. RL74 produced biodiesel more efficiently in palm oil than in soybean oil; and the production was highest at pH 8.0, at 5% methanol and at 20% water content. (C) 2011 Elsevier Ltd. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.