Salmonella typhimurium harboring plasmid expressing interleukin-12 induced attenuation of infection and protective immune responses
- Authors
- Yoon, Won Suck; Choi, Hyuk Jin; Park, Yong Keun
- Issue Date
- 4월-2011
- Publisher
- MICROBIOL RES FOUNDATION
- Keywords
- attenuation; interleukin-12; Salmonella typhimurium; vaccine
- Citation
- JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, v.57, no.2, pp.115 - 122
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
- Volume
- 57
- Number
- 2
- Start Page
- 115
- End Page
- 122
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/112803
- DOI
- 10.2323/jgam.57.115
- ISSN
- 0022-1260
- Abstract
- IL-12 is known to be an essential cytokine which appears to provide protective immunity against intracellular bacteria, such as Salmonella. In this study, we investigated the possibility of developing a vaccine using IL-12 against virulent Salmonella. We used the host defense system activated by cytokine IL-12. The highly virulent Salmonella strain (Salmonella typhimurium UK-1) was transformed with cytokine-expressing plasmids. These live, wild-type pathogens were used as vaccine strains without undergoing any other biological or genetic attenuating processes. The newly developed strains induced partial protection from infections (30-40%). Of note, the interleukin-12-transformed pathogen was safe upon immunization with low doses (10(3) cfu), induced IgG responses, and stimulated protective immune responses against Salmonella typhimurium in mice (80-100%). These results suggest that IL-12 induced attenuation of wild-type Salmonella in the host infection stage and vaccine development using the wild-type strain harboring plasmid-secreting IL-12 may be considered as an alternative process for intracellular bacterial vaccine development without the inconvenience of time-consuming attenuation processes.
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