Soybean (Glycine max L. Merr.) Hexane Extracts Inhibit Cellular Fatty Acid Uptake by Reducing the Expression of Fatty Acid Transporters
- Authors
- Do, Kyoungrok; Jeun, Jungae; Houng, Soung-Jin; Jun, Hee-jin; Lee, Ji Hae; Jia, Yaoyao; Lee, Sung-Joon
- Issue Date
- 2월-2011
- Publisher
- KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
- Keywords
- soybean; fatty acid uptake; fatty acid transport protein-4; CD-36
- Citation
- FOOD SCIENCE AND BIOTECHNOLOGY, v.20, no.1, pp.237 - 242
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- FOOD SCIENCE AND BIOTECHNOLOGY
- Volume
- 20
- Number
- 1
- Start Page
- 237
- End Page
- 242
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/113163
- DOI
- 10.1007/s10068-011-0032-7
- ISSN
- 1226-7708
- Abstract
- Intake of saturated and trans-fatty acids is a strong risk factor for coronary heart disease. We investigated the inhibitory effects of 2 hexane extracts from white (WBE) and black soybeans (BBE) on cellular fatty acid uptake in vitro. Transcellular uptake of elaidic acid (t18:1), a major trans-fatty acid present in processed foods, in Caco-2 monolayers was significantly reduced by 28.3 and 16.7% 60 min after WBE and BBE treatment, respectively. Results of flow cytometry (FACS) analysis showed significant reductions in boron-dipyrromethene (BODIPY) fluorescence-labeled fatty acid uptake by 35.4 and 40.2% with WBE and BBE treatment, respectively. BBE treatment significantly reduced the expression of fatty acid transport protein-4 and CD36 in Caco-2 cells, as determined by quantitative real time-polymerase chain reaction (qRT-PCR). Similar trends were found in WBE treatment, although to a lesser degree. These observations suggest that soybean extract may reduce fatty acid uptake and cellular fat accumulation by altering fatty acid transporter expression.
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