Cancer stem cell traits in squamospheres derived from primary head and neck squamous cell carcinomas
- Authors
- Lim, Young Chang; Oh, Se-Yeong; Cha, Yun Yi; Kim, Sung-Hak; Jin, Xun; Kim, Hyunggee
- Issue Date
- 2월-2011
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Neoplastic stem cells; Head and neck neoplasms; Squamospheres; Oral cancer
- Citation
- ORAL ONCOLOGY, v.47, no.2, pp.83 - 91
- Indexed
- SCIE
SCOPUS
- Journal Title
- ORAL ONCOLOGY
- Volume
- 47
- Number
- 2
- Start Page
- 83
- End Page
- 91
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/113230
- DOI
- 10.1016/j.oraloncology.2010.11.011
- ISSN
- 1368-8375
- Abstract
- A subpopulation of cancer stem cells (CSCs), but not the majority of non-tumorigenic cancer cells, in a variety of human malignancies plays a critical role in cancer cell proliferation, invasion, metastasis, and tumor recurrence post-therapies. We report the isolation of sphere-forming cells (squamospheres) from primary head and neck squamous cell carcinomas (HNSCCs), and characterization of their CSC properties. Squamospheres appeared within 2 weeks after seeding as single-dissociated cells obtained from primary HNSCC specimens in serum-free culture conditions. Real-time RT-PCR and immunocytochemistry assays revealed that a number of stem cell markers, including CK5, OCT4, SOX2, and nestin, were up-regulated in HNSCC-driven squamospheres. Fluorescence-activated cell sorting (FACS) analysis showed that squamospheres contain enriched side population cells compared to serum-induced differentiated squamosphere cells. Furthermore, HNSCC-driven squamospheres appeared to be chemoresistant to cisplatin, 5-fluorouracil (FU), paclitaxel and doxetaxel, and showed increased levels of ABCG2, one of the ATP-binding cassette (ABC) transporters. Of particular interest, in sharp contrast to subcutaneous injection of 1 x 10(6) differentiated squamosphere cells, as few as 100 squamosphere cells were able to give rise to tumors in nude mice. Altogether, we assert that primary HNSCC-driven squamospheres possess CSC properties, and its functional analysis may provide a novel tool for investigating the tumorigenic process of HNSCC. (C) 2010 Elsevier Ltd. All rights reserved.
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Collections - College of Life Sciences and Biotechnology > Division of Biotechnology > 1. Journal Articles
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